| 摘要: |
| 采用实时荧光定量PCR技术分析白斑综合征病毒(WSSV)感染凡纳滨对虾不同时段鳃、肠、肝胰腺、肌肉、类淋巴和血细胞组织中热休克蛋白(HSP)60和90基因mRNA转录水平的变化。结果显示，HSP60和HSP90在这6个组织中都有表达，WSSV感染影响各组织中HSP60和HSP90 mRNA转录水平的表达，WSSV感染抑制HSP60在鳃、肠、肌肉和血细胞的表达，促进HSP90在鳃、肠、肝胰腺、肌肉、血细胞和类淋巴的表达，表明HSP60和HSP90参与WSSV感染免疫反应。 |
| 关键词: 凡纳滨对虾 WSSV 热休克蛋白60 热休克蛋白90 |
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| 基金项目:国家重点基础研究发展计划(2012CB114401)、对虾行业专项（201103034）和国家自然科学基金（30871942） |
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| Expression of HSP60 and HSP90 in shrimp Litopenaeus vannamei during WSSV infection |
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| Abstract: |
| The mRNA expression levels of HSP60 and HSP90 in tissues of gill, intestine, hepatopancreas, muscle, hemocyte and lymphoid were assayed by quantitative real-time PCR. Results showed that HSP60 and HSP90 were expressed in the six tissues. WSSV infection affected the transcription levels of HSPs mRNA. WSSV infection inhibited the expression of HSP60 in the tissues of gill, intestine, muscle and hemocyte, and promoted the expression of HSP90 in the tissues of gill, intestine, hepatopancreas, muscle, hemocyte and lymphoid. This indicated that both HSP60 and HSP90 took part in immune responses. |
| Key words: Litopenaeus vannanei White spot syndrome virus HSP60 HSP90 |
