饲料中维生素C对大菱鲆繁殖性能的影响

Impact of dietary vitamin C on the reproductive performance of turbot Scophthalmus maximus

关键词:  大菱鲆  亲鱼  饲料  维生素C  繁殖性能

Key words:  Turbot Scophthalmus maximus  Broodstock  Diet  Vitamin C  Reproductive performance

摘要:

在基础饲料中分别添加0、800和4 800mg/kg的维生素C饲养大菱鲆亲鱼240d，通过比较各处理组亲鱼的生长、性腺指数、相对产卵量、上浮卵率、孵化率、卵径以及各组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，研究维生素C对大菱鲆亲鱼繁殖性能的影响。实验结果表明，各处理组亲鱼生长无显著差异（P>0.05），但随着饲料中维生素C添加量的增加，亲鱼的生长呈上升趋势。800和4 800mg/kg组亲鱼相对产卵量显著高于对照组（P<0.05）。随着饲料中维生素C添加量的增加，亲鱼性腺指数、产卵次数、上浮卵率、受精率和孵化率均显著提高（P<0.05），亲鱼卵和组织中维生素C含量反映了饲料中的维生素C含量。虽然各处理组亲鱼的卵径和油球径差异均不显著（P>0.05），但随着饲料中维生素C添加量的增加，亲鱼卵径和油球径呈上升的趋势。肝脏、卵巢和血清中SOD活性随饲料中维生素C添加量的增加而显著升高（P<0.05），而MDA含量则显著下降（P<0.05）。综上所述，大菱鲆亲鱼饲料中添加维生素C，能有效改善大菱鲆的繁殖性能。

Abstract:

An eight-month feeding experiment was conducted to determine the impact of dietary vitamin C on the reproductive performance of turbot Scophthalmus maximus.The basal diet was supplemented with 0,800 and 4 800 mg/kg vitamin C to formulate three experimental diets.Results showed that the growth of turbot broodstock was improved with the increasing dietary vitamin C, but no significant difference was observed between control and vitamin C supplementation treatments.The egg production of female broodstock fed vitamin C supplemented diets was significantly (P<0.05) higher than those fed the basal diet.The gonadosomatic index (GSI),number of spawns,percentage of buoyant egg,fertilization rate and hatching rate of 800 mg/kg and 4 800 mg/kg treatments were significantly higher with the increase of vitamin C addition in diets(P<0.05). Vitamin C concentrations in eggs reflected dietary vitamin C addition.The egg diameter and oil globule diameter of turbot broodstock was improved with the increasing dietary vitamin C,but no significant difference was observed between control and vitamin C supplementation treatments.With the increase of dietary vitamin C, the activities of superoxide ismutase (SOD) in tissues were significantly reduced (P<0.05),but malonaldehyde (MDA) contents were significantly increased (P<0.05).So,it is concluded that vitamin C supplement in the diets can improve the reproductive performance of turbot broodstock.