黄姑鱼群体遗传结构的同工酶分析

韩志强; 高天翔; 韩冰; 庄志猛

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黄姑鱼群体遗传结构的同工酶分析
韩志强¹, 高天翔², 韩冰², 庄志猛³
1.1 中国海洋大学海水养殖教育部重点实验室，青岛 266003） （2 中国水产科学研究院黄海水产研究所，青岛 266071;2.1 中国海洋大学海水养殖教育部重点实验室，青岛 266003;3.2 中国水产科学研究院黄海水产研究所，青岛 266071

摘要:

采用水平淀粉凝胶电泳技术对黄姑鱼青岛、舟山和厦门3个群体的遗传结构和遗传分化进行了研究。在G3PDH、IDHP、LDH、MDH、PGDH、PGM、SDH和SOD等 8种酶中，共检测到11个基因位点。3个黄姑鱼群体在G3PDH*和PGM*位点上均呈多态，其多态位点比例均为02。3个群体的平均杂合度观测值和预期值分别在0.010 1-0.037 9和0.023 4-0.039 9之间，平均每个位点的有效等位基因数在1.029 0-1.052 8之间。3个群体间遗传距离和遗传一致度分别为0.000 4-0.000 8和0.999 2-0.999 6。研究结果表明，3个黄姑鱼群体之间无明显的遗传分化

关键词: 黄姑鱼同工酶遗传变异

DOI：

分类号:

基金项目:国家自然科学基金（30471329）、国家重点基础研究发展计划项目（2005CB422306）和国家高新技术发展计划项目（2006AA09Z418）共同资助

Genetic structures of Nibea albiflora as revealed by isozyme analysis

Abstract:

Horizontal starch gel electrophoresis was used to investigate the genetic structure 〖JP2〗and differentiation of the three〖JP〗 populations (Qingdao, Zhoushan and Xiamen) of Nibea albiflora. Eleven putative loci were revealed by eight enzymes (G3PDH, IDHP, LDH, MDH, PGDH, PGM, SDH and SOD). The percentage of polymorphic loci for each population was 02. The mean observed and expected heterozygosity ranged from 0010 1 to 0037 9 and from 0023 4 to 0039 9, respectively. The average number of efficient allele for each locus varied from 1.029 0 to 1.052 8. The genetic distances among populations were from 0.000 4 to 0.000 8, and the genetic similarities were from 0.999 2 to 0.999 6. The results showed that there was no significant genetic differentiation among the three populations of N. albiflora

Key words: Nibea albiflora Isozyme Genetic variation