龙胆石斑鱼源美人鱼发光杆菌的生物学特性与系统发育学分析

Biological characterization and phylogenetic analysis of Photobacterium damselae subsp. damselae from diseased Epinephelus lanceolatus L.

关键词:  龙胆石斑鱼  美人鱼发光杆菌美人鱼亚种  生物学特性  系统发育学

Key words:  Epinephelus lanceolatus L.  Photobacterium damselaesubsp. damselae  Biological characterization  Phylogenetic analysis

摘要:

对从1尾病死的观赏用龙胆石斑鱼Epinephelus lanceolatus L.中分离到的细菌，进行了形态特征、理化特性和对抗菌类药物的敏感性等较系统的表观生物学性状鉴定。同时，测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树。结果表明,供试两株纯培养菌（编号：HQ061227 1、HQ061227 2）为发光杆菌属Photobacterium（Beijerinck〓1889）的美人鱼发光杆菌美人鱼亚种P. damselae subsp. damselae(Love et al.〓 1982;Smith et al.〓1991)，用HQ061227 1株作为代表菌株的16S rRNA基因序列长度（不包括引物结合区）为1 469bp（GenBank 登录号：EF635307），与GenBank数据库中美人鱼发光杆菌美人鱼亚种的同源性在99%。药敏试验结果显示,对供试37种抗菌药物中的青霉素G等4种耐药, 对头孢唑啉等32种敏感, 对氨苄青霉素低敏。

Abstract:

The biological characteristics and phylogenetic analysis of bacteria isolated from Epinephelus lanceolatus L. were studied, including morphological characteristics, physiological and biochemical characteristics. In addition, the 16S rRNA gene were partially sequenced and compared with sequences registered in databases; and molecular phylogenetic trees were constructed. The results showed that the two pure strains （HQ061227 1、HQ061227 2）belonged to P. damselae subsp. damselae (Love et al. 1982; Smith et al. 1991) of Photobacterium (Beijerinck〓1889 ), the sequenced 16S rRNA gene of strain HQ061227 1（accession No. EF635307) is 1 469bp in length, and exhibited high similarity (99%) with the 16S rRNA gene of P. damselae from GenBank database. Antibiotic sensitivity using 37 antimicrobial agents showed that the isolates were sensitive to 32 agents including cefazolin, resistant to 4 agents including penicillin G, and slightly sensitive to ampicillin.