温度和pH值对斜带髭鲷蛋白酶、淀粉酶活性的影响

Effects of temperature and pH on the protease and amylase activities in skewband grunt Hapalogenys nitens

关键词:  斜带髭鲷  温度  pH值  蛋白酶  淀粉酶  活性

Key words:  Skewband grunt Hapalogenys nitens  Temperature  pH  ProteaseAmylase  Activity

摘要:

研究了温度和pH值对斜带髭鲷Hapalogenys nitens蛋白酶、淀粉酶活力的影响。结果表明，斜带髭鲷胃内pH值范围为4.9～5.4；肝胰脏pH值为5.8～6.2；肠道pH值为6.5～6.9。蛋白酶活性随温度的上升而增加，在40 ℃达最大，40 ℃以上酶活性随温度的上升而下降。在不同消化器官中，蛋白酶活性大小顺序为：胃＞前肠＞幽门盲囊＞后肠＞肝胰脏。在15～50 ℃范围内，斜带髭鲷消化道不同部位淀粉酶活性的最适温度均为35 ℃，淀粉酶活性由高到低顺序为:肝胰脏＞幽门盲囊＞前肠＞后肠＞胃。在pH值为2.2～7.6范围内，胃蛋白酶活性的最适pH值为2.8；在pH值为4.8～8.0范围内，其他消化器官蛋白酶的最适pH值均为7.2；在最适pH值下，各消化器官中的蛋白酶活性由高到低顺序为:前肠＞幽门盲囊＞后肠＞肝胰脏。在pH值为4.8～8.0范围内，胃淀粉酶活性的最适pH值为6.0，肠、肝胰脏与幽门盲囊淀粉酶的最适pH值均为68，活性由高到低顺序为:肝胰脏＞幽门盲囊＞前肠＞后肠＞胃。在最适温度和pH值下，蛋白酶活性由高到低顺序为:胃＞前肠＞幽门盲囊＞后肠＞肝胰脏;淀粉酶活性由高到低顺序为:肝胰脏＞幽门盲囊＞前肠＞后肠＞胃。

Abstract:

The effects of temperature and pH on protease and amylase activities in skewband grunt Hapalogenys nitens was studied.Results showed that the pH values of the stomach, hepatopancreas and intestine were 4.9～5.4,5.8～6.2；and 6.5～6.9 respectively. Protease activity rose with the increase of water temperature with a peak at 40 ℃,and then decreased. The order of protease activity was as follows: stomach＞foregut＞pyloric caecum＞hindgut＞hepatopancreas. The optimum temperature for amylase in H. nitens was 35 ℃ within the range of 15～50 ℃; the order of amylase activity was as follows: hepatopancreas＞pyloric caecum＞foregut＞hindgut＞stomach.The optimum pH for protease activity in stomach was 2.8 ℃ within the range of 2.2～7.6, while the optimum pH for protease activity in other digestive organs was 7.2 ℃ with in the range of 4.8～8.0. Under the optimum pH conditions, the order of protease activity in different organs was as follows: foregut＞pyloric caecum＞hindgut＞hepatopancreas. The optimum pH for protease activity in stomach was 6.0 in the range of 4.8～8.0. The optimum pH for amylase activity in stomach was 6.0 while it was 6.8 in intestine, hepatopancreas and pyloric caecum. The order of amylase activity was as follows: hepatopancreas＞pyloric caecum＞ foregut＞hindgut＞stomach.Under the optimum temperature and pH conditions, the order of protease activity was as follows: stomach＞foregut＞pyloric caecum＞hindgut＞hepatopancreas, while that of amylase activity was: hepatopancreas＞pyloric caecum＞foregut＞hindgut＞stomach.