MS-222对牙鲆麻醉效果

Anaesthetic effect of MS-222 on flounder Paralichthys olivaceus

关键词:  牙鲆  麻醉剂MS-222  理想麻醉剂浓度  复苏

Key words:  Flounder Paralichthys olivaceus  Tricaine methanesulphonate (MS-222)  Concentration  Recovery

摘要:

以MS-222为麻醉剂，在13-14 ℃水温条件下，用浸浴麻醉方法测试了不同麻醉剂浓度和不同麻醉时间对牙鲆复苏时间、复苏率和24 h存活率及长时间麻醉对复苏率的影响以及超有效浓度麻醉剂（60 mg/L）麻醉后的抗露干能力。预备实验证明，本试验条件下麻醉剂的有效浓度为50 mg/L。实验表明，将麻醉剂浓度从50 mg/L提高到200 mg/L，供试鱼被麻醉的时间由288 s缩短至50 s，复苏时间由94 s延长至106 s；用60、100和200 mg/L的麻醉液持续麻醉后，可获得100%复苏率的最长麻醉时间分别为64、32和32 min，牙鲆经MS-222麻醉后抗露干时间延长。牙鲆麻醉后复苏过程中存在较大的个体差异，但一旦复苏，牙鲆24 h内的存活率可达100％。实验表明，MS-222的合理麻醉浓度为60 mg/L，此浓度下最长麻醉时间为64 min，麻醉露干时间应低于60 min。

Abstract:

A wide variety of compounds were used to anaesthetize fish in fisheries research, fisheries management, aquaculture and fish health. Among of them, tricaine mesilate ( MS-222) is one of the anaesthetics widely used in the world for it's safety and high effectiveness. In China, little published information was found on the use of MS 222 in marine fish. In this study, immersion anaesthesia was utilized to investigate the recovery time, recovery rate and survival rate in 24 h of flounder Paralichthys olivaceus after being anaesthetized with different concentrations of MS 222 at the temperature of 13-14 ℃; and the effect of long time immersion anaesthesia in 60 mg/L MS-222 on the recovery rate and survival rate after being transferred from water into air were studied. The results showed that the lowest effective concentration to anaesthetize the flounder was at 50 mg/L. The time to achieve anaesthesia was shortened from 288 s to 50 s and the time to recovery was extended from 94 s to 106 s when the concentration of MS-222 was increased from 50 mg/L to 200 mg/L. Three concentrations of MS-222 including 60 mg/L, 100 mg/L and 200 mg/L were used for long time immersion anaesthesia respectively to evaluate the recovery time, recovery rate and survival rate in 24 h, the results showed that the recovery time in the three groups were prolonged along with the increasing of immersion time, but the longest immersion time to achieve 100 percent recovery rate were 64 min, 32 min and 32 min respectively. Also the time for enduring air exposure was tested after the flounder was exposed in 60 mg/L MS 222, and it was found that their ability to endure air exposure was better than the control. Recovery time showed great difference between individuals and the survival rate in 24h after anaesthesia was 100% once the flounder was recovered. These results indicated that the ideal concentration of MS-222 is 60 mg/L, the time to keep the flounder in MS 222 and the exposed time to air is less than 64 s and 60 min respectively at this concentration.