罗氏沼虾成虾中氨基脲的分布特征

Distribution Characteristics of Semicarbazone in Adult Macrobrachium rosenbergii

中文关键词: 罗氏沼虾  氨基脲  呋喃西林  分布特征

英文关键词: Macrobrachium rosenbergii  Semicarbazide  Nitrofurazone  Distribution characteristics

中文摘要:

      从未使用呋喃西林的罗氏沼虾(Macrobrachium rosenbergii)养殖场采集商品规格虾样品，分离壳、肌肉、肌肉表皮层、内脏和复眼等组织测定氨基脲(semicarbazide, SEM)，探究其在商品规格罗氏沼虾不同组织中的本底含量，为质量安全监管提供技术支撑。结果显示，罗氏沼虾各组织中SEM平均含量为(12.90±2.47) μg/kg，其中，外骨骼(复眼、外壳和肢足)中的平均含量分别为(33.29±3.06)、(29.00±5.67)和(28.10±7.08) μg/kg，占总量的77.9%。肌肉中SEM含量最低，平均值为(1.83±0.24) μg/kg。不同组织中SEM含量从高到低依次为复眼>外壳>肢足>肠>鳃>肌肉表皮层>肝>生殖腺>肌肉。鉴于目前的技术手段还难以区分罗氏沼虾体内SEM的来源是内源性还是外源性，因此，建议监管部门在开展罗氏沼虾中呋喃西林残留量的监控时，考虑减去SEM的本底含量，以避免误判情形的发生。

英文摘要:

      As the most stable metabolite of nitrofurazone (NFZ), semicarbazide (SEM) can be determined after the administration of NFZ in aquatic animals within a few hours. Because of extensive tissue distribution and a long residual period, SEM is used as the exclusive marker for NFZ. However, SEM is naturally present in the shells of crustaceans, such as crayfish, shrimp, prawns, and soft-shell crabs. To avoid false-positive results induced by SEM alone and to precisely identify the illegal use of NFZ, further investigations on the distribution characteristics of SEM in crustaceans is essential. In the present study, samples of shell, muscle, muscle epidermis, internal organs, and compound eye were collected from Macrobrachium rosenbergii shrimp farms that were not exposed to nitrofural, and SEM was determined in order to investigate the background concentrations in different tissues of the commercial shrimp. Samples were evaluated in accordance with Announcement No.783-1-2006 of the Ministry of Agriculture of the People’s Republic of China. As multiple tissue samples, including compound eye, outer shell, limbs, feet, intestine, gill, muscle epidermis, liver, gonad, and muscle were assessed, the method was suitable for monitoring the background concentration of SEM in different tissues of M. rosenbergii. The average concentration of SEM across all tissues was (12.90±2.47) μg/kg. The average concentrations of SEM in compound eyes, shells, and limbs were (33.29±3.06), (29.00±5.67), and (28.10±7.08) μg/kg, respectively. The lowest concentration of SEM was found in muscle samples, with an average value of (1.83±0.24) μg/kg. The concentration of SEM in commercial M. rosenbergii tissues could be ordered as compound eye > outer shell > limbs and feet > intestine > gill > muscle epidermis > liver > gonad > muscle. Considering that it was difficult to distinguish the source of SEM in M. rosenbergii, the background concentration of SEM should be deducted when determining NFZ residues in this species.