热带糙海参雌、雄亲参的差异代谢物和差异代谢通路研究

Study on sex differential metabolites and metabolic pathway of parental tropical sea cucumbers Holothuria scabra

中文关键词: 糙海参  LC-MS/MS  体腔液  性别差异代谢物  性别差异代谢通路

英文关键词: Holothuria scabra  LC-MS/MS  Coelomic fluid  Sex differential metabolites  Sex differential metabolic pathway

中文摘要:

      本研究基于LC-MS/MS代谢物分析方法，探究了糙海参(Holothuria scabra)体腔液中性别特异性代谢物，同时分析了代谢通路的性别差异。结果显示，与雄性糙海参(M)相比，在雌性糙海参(F)的体腔液中共筛选出1352个上调代谢物和943个下调代谢物。根据数据库比对和注释，共筛选出66个具有显著差异的代谢物(P≤0.05, VIP≥1)，其中，β-胡萝卜素具有作为性别差异代谢标志物的潜力。结果还显示，体腔液中会积累其他器官需求较少的代谢产物，如亚精胺在雌性中浓度较高，而脂肪酸在雄性中种类多且含量较高，这些产物可能在体腔液中发挥其他生理功能。另外发现，雄性体腔液倾向于积累外源物质。通过KEGG进行的代谢通路分析表明，雄性糙海参的性腺发育相关通路——谷胱甘肽代谢、精氨酸脯氨酸代谢、甾类化合物生物合成和酪氨酸代谢均发生在体腔液中；雌、雄糙海参性腺发育受到性激素影响，这种影响可能受到多巴胺的调节并具有性别差异。

英文摘要:

      In order to clarify the differences in metabolism between female and male relatives of tropical sea cucumbers (Holothuria scabra), the sex-specific metabolites in their coelomic fluid were explored by a liquid chromatography-tandem mass spectrometry metabolite analysis, and the sex-specific metabolites and pathways were analyzed. The results showed that compared with male sea cucumbers, a total of 1352 up-regulated metabolites and 943 down-regulated metabolites were screened in the coelomic fluid of female sea cucumbers. According to a database comparison and annotation, a total of 66 metabolites with significant differences (P≤0.05, VIP≥1) were selected. Among them, β-carotene has the potential to be a gender difference metabolic marker. Spermidine and fatty acids that are less demanded by other organs were found at higher concentrations in females and males, respectively. We speculated that these compounds may be used by other physiological functions. In addition, it was also found that male coelomic fluid tended to accumulate xenobiotics. Metabolic pathway analysis by KEGG showed that the gonadal development-related pathways, glutathione metabolism, arginine and proline metabolism, steroid biosynthesis, and tyrosine metabolism of male sea cucumbers all occurred in the coelomic fluid. The gonadal development of female and male sea cucumbers was affected by sex hormones, which may be regulated by dopamine and have gender differences.