凡纳滨对虾SNP标记开发与家系亲缘关系验证分析

Development of SNP markers and verification analysis of relationship on family in Litopenaeus vannamei

中文关键词: 家系  SNP标记  遗传多样性  聚类分析  亲子鉴定

英文关键词: Family  SNP markers  Genetic diversity  Cluster analysis  Parentage assignment

中文摘要:

      本研究基于转录组序列开发了37个SNP标记，并对22个凡纳滨对虾(Litopenaeus vannamei)家系进行了遗传多样性、家系聚类和亲子鉴定分析，探讨SNP标记在对虾家系选择育种中的应用途径。结果显示，37个SNP位点在凡纳滨对虾22个家系中的平均期望杂合度(He)和平均观测杂合度(Ho)分别为0.38和0.34；平均多态信息含量(PIC)为0.30，属于中度多态性(0.25

英文摘要:

      The Large-scale family-based selective breeding can effectively increase genetic gain in shrimp, but tagging individuals for tracking pedigree is time-consuming, labor-intensive, and would cause inaccurate kinship. Molecular markers are effective in solving this problem. In this study, we developed 37 SNP markers derived from transcriptome sequences and performed analysis on genetic diversity, family clustering, and parentage for 22 families of Litopenaeus vannamei. The results provided examples for the application of SNP markers in selective breeding of shrimp. The results showed that the average expected heterozygosity (He) and observed heterozygosity (Ho) for 37 SNP loci were 0.38 and 0.34, respectively. Further, the average polymorphism information content (PIC) was 0.30, which means moderate polymorphism (0.25