| In order to explore the molecular mechanism of high-temperature tolerance in turbot, Scophthalmus maximus L., and to screen heat tolerance related genes, the IlluminaHiSeq-2500 platform was used to sequence the transcriptome of five turbot kidneys from five different heat treatments, through bioinformatics analysis, including GO (gene function annotation), SSR (simple repeat sequence) analysis, etc. The main results obtained were as follows: the total number of Unigenes obtained by assembly was 68525, the length range was 201~23456 bp, the average length was 1124 bp, and the length of N50 was 2316 bp. Sequence alignment and function annotation of Unigenes was conducted in Nr, Swissprot, KEGG, KOG, and GO databases. A total of 25,498 clusters were obtained, of which the Nr database noted the most Unigenes; and according to GO function classification, had three kinds of cell components, molecular functions, and biological processes, including 56 functional groups. A large number of Unigenes were related to cell processes, metabolic processes, catalytic activities, biological regulation, and stress response. Further, Unigenes were classified into 218 metabolic pathways and five KEGG pathways: metabolic pathway, genetic information processing, cellular processes, environmental information, and biological systems. A total of 65 transcription factors were detected by transcription factor analysis, with the C2H2 Zn finger protein family having the highest number of genes. Through the analysis of gene expression profiles under different temperature stress, there were significant differences among different temperature groups, In the 20℃ and 28℃ groups, the difference was the largest; 4734 genes, of which 3386 were up-regulated and 1348 were down-regulated. In this study, we established a database of renal transcriptional groups in turbot under heat stress, which provides abundant data for the study of molecular mechanisms of heat stress in turbot. |
