LED光谱对红鳍东方鲀仔稚鱼形态性状及生长相关基因表达的影响

Effects of LED spectra on morphological character and gene expression of growth in Takifugu rubripes larvae

中文关键词: LED光谱  红鳍东方鲀仔稚鱼  形态性状  生长相关基因的表达

英文关键词: LED Spectrum  Takifugu rubripes larvae  Morphological character  Gene expression of growth

中文摘要:

      设置4种LED光谱环境，分别为蓝(λ450 nm)、绿(λ525 nm)、黄(λ590 nm)和白(λ400~780n m)，水温控制在(22±1)℃，光周期为16L:8D，光强设为200 mW/m2。研究光谱对红鳍东方鲀(Takifugu rubripes)受精卵孵化和孵化后1~18 d (即实验第6~23天)仔稚鱼形态性状(全长、体长、躯干长、尾长、头长、眼径和体高)以及生长相关基因，包括生长激素(GH)、生长激素受体1型(GHR1)、类胰岛素生长因子1型(IGF1)基因相对表达量的影响。结果显示，蓝、绿、黄光处理组的受精卵比白光处理组早2 d孵化；在实验结束时，蓝光处理组中，仔稚鱼的全长、体长、躯干长和尾长生长最快，黄光处理组中，头长、眼径和体高生长最快。对于生长相关基因，蓝光下的GH基因表达量显著高于黄、白光组(P<0.05)，但与绿光组无显著性差异；不同光谱下，GHR1、IGF1基因的表达量无显著性差异。结果表明，蓝光、绿光和黄光促进红鳍东方鲀受精卵的孵化，蓝光有利于红鳍东方鲀仔稚鱼的生长发育。本文研究了不同光谱对红鳍东方鲀仔稚鱼早期发育的形态性状及生长相关基因表达的影响，为养殖厂对红鳍东方鲀育苗提供科学的参考依据。

英文摘要:

      Four LED spectral parameters were set, which were blue (λ450 nm), green (λ525 nm), yellow (λ590 nm), and white (λ400~780 nm), water temperature was controlled at (22±1)℃, photoperiod was 16L:8D. The light intensity was set at 200 mW/m2. The results showed that the fertilized eggs of Takifugu rubripes under the blue, green and yellow light hatched 2 days earlier than the white light. At the end of the experiment, the full length, body length, trunk length and tail length of the larvae under the blue light were the most. Under yellow light, the head length, eye diameter, and body height showed the fastest growth. For growth genes, the expression of growth hormone (GH) gene under the blue light was significantly higher than yellow and white light group (P<0.05), but there was no significant difference with green light group. There was no significant difference in gene expression for growth hormone receptor 1 (GHR1) and insulin-like growth factor type (IGF-1) under different spectra, but the expression of GH gene under blue light was the highest. The results showed that LED spectrum (blue, green, and yellow) promoted the hatching of the fertilized eggs of T. rubripes, and the blue spectrum is beneficial to the growth and development of the T. rubripes larvae. The paper provided a scientific reference for the incubation of T. rubripes.