基于耳石δ13C和δ18O的黄、渤海秋季小黄鱼补充群体的种群划分

Investigating the Population Structure of Larimichthys polyactis from the Yellow Sea and Bohai Sea Using Stable Isotope Mass Spectrometry

中文关键词: 耳石  δ13C  δ18O  小黄鱼  种群划分

英文关键词: Otolith  δ13C  δ18O  Larimichthys polyactis  Population classification

中文摘要:

      关于小黄鱼的种群划分问题，从20世纪50年代至今一直存在争议。以往研究海洋鱼类种群划分的方法主要有标记重捕法、渔获量分析法、寄生虫标记法、形态表型量度特征法、分子生物学法和钙质结构元素指纹法等，而这些方法在细节的表述方面有各自的局限性。本研究采用稳定同位素质谱分析技术，分析了黄、渤海渔业资源调查中92尾小黄鱼(Larimichthys polyactis)的左矢耳石样品中δ13C和δ18O的值，并对每尾小黄鱼耳石δ13C和δ18O的值进行聚类分析。结果显示，我国黄、渤海秋季小黄鱼早期补充群体可划分为4个种群，即渤海黄海北部种群、黄海中部种群、黄海南部离岸种群和黄海南部沿岸种群。其中，黄海南部离岸种群和其他3个种群没有站位交叉现象。对聚类分析的结果进行判别分析，得出渤海黄海北部种群的判别成功率为75.9%，黄海中部种群的判别成功率为80.0%，黄海南部离岸种群判别成功率为81.0%，黄海南部沿岸种群判别成功率为95.5%，总体判别成功率为82.6%。首次将黄海南部种群细分为了黄海南部离岸种群和黄海南部沿岸种群，且2种群之间很少有站位的交叉现象。

英文摘要:

      The issue of population division of the small yellow croaker (Larimichthys polyactis) has been controversial since the 1950s. Previously used methods for studying the population classification of these marine fish populations include the mark-recapture method, catch yield statistics method, parasite labeling method, morphometric/meristic characters method, molecular biology method, and chemical fingerprints of the calcified structures method. Each of these techniques has its own reliability as well as limitations. In this study, the content of delta 13C (δ13C) and delta 18O (δ18O) in the left sagittal otolith samples of 92 Larimichthys polyactis obtained from the investigation of fishery resources in Bohai Sea and Yellow Sea, was determined using stable isotope mass spectrometry. A cluster analysis was carried out based on differences in the content of delta 13C and delta 18O among samples. The results showed that the early supplement population of Larimichthys polyactis, predominant during the autumn season, in the Yellow Sea and Bohai Sea can be divided into four major groups, which are listed as Bohai and the northern Yellow Sea population, the central Yellow Sea population, the offshore population of southern Yellow Sea, and the coastal population of southern Yellow Sea. However, there was no station between the offshore population of the southern Yellow Sea and the three other populations in the Yellow and Bohai Sea. The discriminant and cluster analysis revealed that the successful rate of discrimination was 75.9%, 80.0%, 81.0%, and 95.6% in the Bohai and northern Yellow Sea population, the central Yellow Sea population, the offshore population of southern Yellow Sea, and the coastal population of southern Yellow Sea, respectively. The overall successful rate of discrimination was recorded to be 82.6%. The population of southern Yellow Sea was subdivided into offshore and coastal populations for the first time, and no station crossing between the two populations was recorded. With this study, we propose to establish the basis for population classification of Larimichthys polyactis.