南极磷虾油中砷甜菜碱在大鼠体内的代谢

The Metabolism of Arsenobetaine in Antarctic Krill Oil in Rats

中文关键词: 南极磷虾油  砷甜菜碱  代谢  蓄积

英文关键词: Antarctic krill oil  Arsenobetaine  Metabolism  Cumulative effect

中文摘要:

      为研究南极磷虾(Euphausia superba)油中的砷甜菜碱在大鼠体内的吸收、分布和蓄积情况，随机将雄性Wistar大鼠分为3组：大豆油对照组、磷虾油组、添加100 mg/kg砷甜菜碱的磷虾油组，灌胃剂量为2.625 g油/kg·bw。于灌胃后1、2、4、6、12、24 h，经戊巴比妥钠麻醉，腹主动脉取血处死大鼠，并收集12 h和24 h尿液，ICP-MS法检测血浆、肝脏、肾脏、脾和尿液中总砷含量。另连续灌胃同等剂量油脂7 d和30 d，检测肝脏和肾脏中总砷含量。结果显示，磷虾油组和对照组大鼠血浆和脏器中总砷含量各时间点无显著变化；砷甜菜碱磷虾油组大鼠血浆中总砷含量于1 h达到最高，脏器中总砷含量均在6 h达到最高，24 h后降低至最高值的30%以下；85%的砷会在24 h之内随尿液排出体外。7 d和30 d连续灌胃后，2个实验组大鼠脏器中总砷含量较对照组均无显著变化。南极磷虾油中的砷甜菜碱在大鼠体内很少蓄积，大部分于24 h内随尿液排出体外，而长期摄食也未出现脏器蓄积现象。本研究对于指导南极磷虾油及其相关产品的科学消费和拓展应用具有重要意义。

英文摘要:

      To study the absorption, distribution, and accumulation of arsenobetaine in Antarctic krill (Euphausia superba) oil, Wistar rats were randomly divided into 3 groups and treated with 2.625 g/kg·bw of soybean oil, krill oil, or 100 mg/kg arsenobetaine added to krill oil. The plasma, liver, kidney and spleen of the rats were collected at 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h after gavage, whereas the urine was also collected at 12 h and 24 h. The total arsenic content in the plasma, organs, and urine was detected by ICP-MS. The total arsenic contents in liver and kidney of rats were also measured at 7 d and 30 d after continuous gavage with the same dose of the lipids. The total arsenic contents in the plasma and organs showed no obvious changes at each time point between the krill oil group and the control group. The maximum content of total arsenic in plasma occurred at 1 h after gavage, whereas the total arsenic content in the liver, kidney, and spleen were all the highest at 6 h after gavage. At 24 h after gavage, the content of total arsenic in the liver, kidney, and spleen decreased to below 30% of the maximum. Within 24 h, 85% of the arsenic in the krill oil was passed out of the body in the urine. After continuous gavage for 7 days and 30 days, the total arsenic contents in the liver and kidney of the rats in each group showed no significant changes when compared with those of the control group. Arsenobetaine in the krill oil was rarely absorbed in rats and most passed out of the body in the urine within 24 h. No cumulative effects appeared after long-term feeding with the krill oil and the arsenobetaine.