刺参(Apostichopus japonicus) 4个群体棘数的重复力估计

Estimates of Repeatability Coefficients for Papillae Number in Four Populations of Sea Cucumber (Apostichopus japonicus)

中文关键词: 刺参  棘数  重复力  选择育种

英文关键词: Apostichopus japonicus  Papillae number  Repeatability  Selective breeding

中文摘要:

      为了研究刺参(Apostichopus japonicus)棘数的重复力、棘数所需度量次数和棘数最大可能生产力(MPPA)，本研究针对韩国自繁群体(KK)、多刺自繁群体(DD)、山东自繁群体(SS)和三元杂交群体(DK) 4个刺参群体，采用数量遗传学方法估计了12–17月龄刺参棘数的重复力，计算并比较了4个群体棘数所需的度量次数、MPPA及其与刺参体重的相关系数。结果显示，在12–17月龄时，4个群体刺参棘数的重复力从高到低依次为DD(0.29)>DK(0.28)>SS(0.20)>KK(0.19)，且均为低度重复力；对DD和DK群体棘数测量5次，测量相对准确率可达80%以上，而SS和KK群体则需要测量8次才能达到80%以上准确率；4个群体刺参棘数的MPPA从高到低依次为DD(41.3)> DK(40.8)>KK(39.8)>SS(39.1)，但仅DD和SS群体间存在显著差异(P<0.05)；各群体内刺参棘数MPPA与体重均呈现显著的表型正相关(P<0.05)。以棘数作为育种目标性状，其重复力和MPPA可作为选择群体的依据。研究表明，DD和DK群体的棘数多于另外2个群体，并且具有更高的选育潜力，更利于性状测定。因此，可作为重点群体进行进一步选育。

英文摘要:

      Sea cucumber (Apostichopus japonicus) has become one of the major aquacultural species in China. Genetic improved seeds of sea cucumber play a key role in the ever-increasing profit. Information on the genetic parameters of papillae number, the important economic trait of sea cucumber, however, has been lacking. To investigate the repeatability, required measure times, and the most probable producing ability (MPPA) of sea cucumber, we applied quantitative genetic methods to determine the repeatability coefficients of papillae number of four sea cucumber populations in the age of 12–17 month, including Korean self-propagated population (KK), Duoci self-propagated population (DD), Shandong self-propagated population (SS), and Three-way cross population (DK). Based on the estimates, we also calculated and compared the required measure times, MPPA, and correlation efficient between MPPA and body weight of the four populations. We found that the repeatability coefficients of papillae number were low in all four populations. The repeatability coefficients of the four population followed the order: DD(0.29)>DK(0.28)>SS(0.20)>KK(0.19). For population DD and DK, the papillae number needed to be measured for 5 times to reach a relative accuracy above 80%, while for SS and KK populations 8 measure times were required. The MPPA for papillae number followed the order: DD(41.3)>DK(40.8)> KK(39.8)> SS(39.1), with the significant difference between DD and SS only (P<0.05). The phenotype correlation between MPPA and body weight in each population was significantly positive. Therefore, repeatability and MPPA could be used as criteria in population selection for papillae number. Our results suggested that DD and DK populations might have higher papillae numbers, higher potential for selection, and less measure times needed than the other two populations, hence they could be the key populations for papillae number selection.