饲料中添加南极磷虾粉对半滑舌鳎(Cynoglossus semilaevi)雄性亲鱼繁殖性能及抗氧化功能的影响

Effects of Dietary Antarctic Krill on the Reproductive Performance and Antioxidant Function in Male Broodstock Tongue Sole (Cynoglossus semilaevis)

中文关键词: 南极磷虾粉  半滑舌鳎  雄性亲鱼  繁殖性能  抗氧化功能

英文关键词: Antarctic krill meal  Cynoglossus semilaevis  Male broodstock  Reproductive performance  Antioxidant function

中文摘要:

      饲料中添加0、10%、20%的南极磷虾粉，配制3组(对照组K-0、K-10、K-20)等氮等脂的实验饲料，在室内流水养殖系统进行为期90 d的养殖实验，探讨南极磷虾粉对半滑舌鳎(Cynoglossus semilaevis)雄鱼繁殖性能和抗氧化能力的影响。结果显示，饲料中添加南极磷虾粉对半滑舌鳎雄鱼肝体比、性体比、精液浓度、睾酮含量均无显著性影响(P>0.05)；K-10组与K-20组精巢中超氧化物歧化酶(SOD)活性无显著性差异(P>0.05)，但均显著高于对照组(P<0.05)，K-10组与K-20组精巢、血清和肝脏中的丙二醛(MDA)含量无显著性差异(P>0.05)，但均显著低于对照组(P<0.05)。在本实验条件下，饲料中添加南极磷虾粉不能显著提高半滑舌鳎雄鱼的繁殖性能(P>0.05)，但能显著提高雄鱼的抗氧化功能(P<0.05)。

英文摘要:

      The tongue sole C. semilaevis is a flatfish with high economic value and is extensively exploited in northern China. However, broodstock nutrition of this species is still based on a diet of fresh feeds, which has an important sanitary risk, and contributes to the deterioration of water quality. Therefore, a 90 days feeding trial was conducted to investigate the effect of dietary Antarctic krill meal in broodstock diets on reproductive performance and antioxidant function of male tongue sole Cynoglossus semilaevis. Three diets were formulated to contain respectively 0, 10%, 20% Antarctic krill meals. Three-year-old tongue sole C. semilaevis were stocked in concrete tanks (25 m3) and acclimated to laboratory conditions for 7 days. Then they were randomly divided into three groups, each group had three polyethylene tanks (diameter: 230 cm, high: 100 cm), and they were fed to apparent satiation twice per day for 12 weeks. Results showed that, the hepatosomatic index, sex index, sperm concentration and testosterone contents had no significant differences on each experimental groups (P>0.05). The superoxide dismutase (SOD) activities in semen and eggs had no significant differences between K-10 and K-20 group (P>0.05), but were significantly higher than that of the control group (P<0.05); The malondialdehyde (MDA) content in semen, serum and liver had no significant differences between K-10 and K-20 group (P>0.05), but were significantly lower than that of the control group (P<0.05). In conclusion, the present study suggested that dietary Antarctic krill meal had no significant effect on reproductive performance (P>0.05), but can significant improve antioxidant function for male C. semilaevis (P<0.05).