海萝(Gloiopeltis furcata)藻体保存和容器内的培养方法

Studies on Culture and Storage of Gloiopeltis furcata Thalli

中文关键词: 海萝  藻体  规格  培养  容器  保存

英文关键词: Gloiopeltis furcata  Thalli  Size  Culture  Container  Storage

中文摘要:

      为探索海萝(Gloiopeltis furcata)藻体的陆地工厂化养殖，分三部分实验进行研究：(1)不同长度(0.5、1、1.5 cm)和断开部位(从基部断开、从顶部断开和从藻体分叉处断开)的海萝藻体培养；(2)不同培养密度(0.5、1、2、4 g/L)和培养容器(方形水槽、圆柱形透明塑料薄膜容器和亚克力筒)；(3)不同风干方式(自然风干、培养箱风干和空调加风扇吹干)和保存温度(4、–18℃)对保存后藻体存活率的影响。结果显示，不同断开部位对相同长度的藻体生长影响不显著(P>0.05)；较小藻体(0.5 cm)的生长速率显著低于其他各组(1.0、1.5 cm) (P<0.05)；方形水槽中海萝藻体的生长速率显著低于亚克力筒和塑料薄膜装置中(P<0.05)；随着培养密度的增加，海萝的日特定生长率呈显著的下降趋势(P<0.05)；以空调加风扇方式进行风干的藻体成活率最高(P<0.05)，在-18℃保存6个月，其成活率为93.8%；在4℃下保存1个月，各组藻体的成活率都为0。较好的条件组合为：进行陆地工厂化养殖的海萝规格应大于0.5 cm，合适的培养密度范围为0.5-1 g/L；成本低且高效的塑料薄膜装置是海萝藻体培养的适宜容器；采用保存海萝藻体于-18℃的方法可使海萝陆地工厂化养殖或相关研究不受制于海萝生长季节，可按需要进行。

英文摘要:

      Three experiments were conducted in this study to explore proper on-land culture conditions for Gloiopeltis furcata thalli. In the first experiment vegetable fragments with different sizes (0.5cm, 1cm, and 1.5cm) and cut parts (cut from base, top, and fork) were incubated in lab for 60 days. In the second experiment G. furcata thalli were incubated in different containers (square tank, acrylic cylinder, and plastic membrane cylinder), and with different densities (0.5 g/L, 1 g/L, 2 g/L, and 4 g/L). In the third experiment G. furcata thalli dried in different ways (indoor wind, artificial climate incubation chambers, air-conditioning and fan) were stored at different temperatures (4℃ and –18℃). We found that with a certain size there was no significant difference in the growth between vegetable fragments from various cut parts. Vegetable fragments with a length of 0.5 cm grew slower than those with other lengths (1.0 cm and 1.5 cm, P<0.05). The SGR (specific growth rate) of G. furcata thalli was the lowest in the square tank (P<0.05), and was decreased along with the increase in culture density (P<0.05). The survival rate was higher in G. furcata thalli dried with air-conditioning and fan (P<0.05) and was 93.8% after preserved at –18℃ for 6 months. However, the survival rate of all groups decreased to 0 in 1 month after stored at 4℃. The results indicated that appropriate on-land culture conditions included reasonable size of vegetable fragments (> 0.5 cm), density between 0.5 g/L and 1 g/L, and plastic membrane cylinder as the container. Vegetable fragments for culture can be readily obtained if preserved at –18℃