恩诺沙星和环丙沙星在泥鳅(Misgurnus anguillicaudatus)体内的残留和消除规律

The Residues and Elimination of Enrofloxacin and Ciprofloxacin in Loach Misgurnus anguillicaudatus

中文关键词: 恩诺沙星  环丙沙星  泥鳅  残留  消除

英文关键词: Enrofloxacin  Ciprofloxacin  Misgurnus anguillicaudatus  Residue  Elimination

中文摘要:

      通过研究恩诺沙星与环丙沙星在泥鳅体内的残留和消除规律，为水产养殖业提供合理的给药方案和休药期。试验前，从养殖场购买的泥鳅在试验水质条件下暂养7 d后，分成3组投放入不同水温的养殖系统中。以恩诺沙星与环丙沙星作为目标化合物，以拌料给药的方式，按20 mg/kg鱼体重的剂量每天投喂1次，连续投喂7 d后，对15、20、25℃ 3种水温条件下，目标化合物在泥鳅体内的残留和消除规律进行了研究。试验结果显示，恩诺沙星与环丙沙星均按一级动力学过程从泥鳅体内消除，且水温对喹诺酮类药物的代谢衰减消除速率有很大影响，在25、20、15℃水温条件下，泥鳅体内的恩诺沙星代谢至10.0 μg/kg以下分别需要25、32、50 d，环丙沙星则分别需要27、31、33 d。由于徐州及周边地区的泥鳅主要输往韩国，其对喹诺酮类药物的限量要求是不超过10 μg/kg，因此，在本试验条件下，建议合理的休药期应不少于32 d。

英文摘要:

      In recent years, with the rigorous limits on drug residues in the loach Misgurnus anguillicaudatus from importing countries, the M. anguillicaudatus exporting enterprises and the export inspections are facing enormous challenges in drug use and control. The current study investigated the residues and elimination of enrofloxacin (ENR) and ciprofloxacin (CIP) in M. anguillicaudatus to provide a reasonable dosing plan and withdrawal period for the aquaculture industry. The loach M. anguillicaudatus purchased from an aquaculture farm were fed 7 days to adapt the experimental water quality and then divided into three groups at different temperatures, and fed with enrofloxacin or ciprofloxacin at a dosage of 20 mg/kg per day for 7 consecutive days. Samples were collected at various time points to measure the contents of ENR and CIP by the high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The results showed that both the elimination of ENR and CIP were complied with the first-order kinetics, and the water temperature significantly affected the elimination rate of both drugs. At 25℃, 20℃ and 15℃, it took 25, 32 and 50 days, respectively, for the enrofloxacin compound metabolized to less than 10.0 μg/kg in the body of M. anguillicaudatus. It took 27, 31 and 33 days for ciprofloxacin to metabolize to less than 10.0 μg/kg. Based on the South Korea limits of no more than 10 μg/kg for quinolones in M. anguillicaudatus, a withdrawal period of 32 days is recommended for the M. anguillicaudatus exporters by considering the actual culturing environments in Xuzhou and nearby area.