饲料中添加不同水平的维生素E对半滑舌鳎(Cynoglossus semilaevis)亲鱼繁殖性能及后代质量的影响

Effects of Different Levels of Dietary Vitamin E on the Reproductive Performance and Offspring Quality of Tongue Sole Cynoglossus semilaevis

中文关键词: 半滑舌鳎  亲鱼  维生素E  繁殖性能  卵子质量

英文关键词: Cynoglossus semilaevis  Broodstock  Vitamin E  Reproductive performance  Egg quality

中文摘要:

      在饲料中分别添加0、200、1200 mg/kg的维生素E (DL-α-生育酚乙酸脂形式)配制成Ec、El和Eh三种试验饲料，饲喂3龄半滑舌鳎亲鱼(雌性1.58±0.19 kg/尾、雄性0.20±0.05 kg/尾) 70 d，探讨饲料中添加不同水平的维生素E对半滑舌鳎亲鱼繁殖性能的影响。结果显示，饲料中添加1200 mg/kg的维生素E (Eh组)不仅可以促进亲鱼性腺的发育，提高亲鱼产卵量、浮卵率、受精率、孵化率以及初孵仔鱼长度，而且影响仔鱼质量，仔鱼的成活率、12 d仔鱼长度、生存活力等方面均要高于对照组。而饲料中添加200 mg/kg的维生素E (El组)只对亲鱼性腺的发育和初孵仔鱼长度有一定的促进作用，对亲鱼产卵量、浮卵率、受精率、孵化率没有显著影响(P>0.05)。随饲料中维生素E的增加，血清中SOD的活性显著升高(P<0.05)，MDA含量则显著下降(P<0.05)。此外，El和Eh试验组卵中干物质、维生素C的含量，以及卵子脂肪酸中PUFA、n-3PUFA、n-3HUFA和n-6PUFA的含量均要高于Ec组，El和Eh试验组之间的没有显著性差异(P>0.05)。结果表明，在半滑舌鳎亲鱼饲料中添加适量维生素E会亲鱼促进性腺的发育，增加产卵量，提高卵子和仔鱼的质量。在本试验中Eh组(1200 mg/kg)亲鱼的繁殖性能要优于Ec组(0 mg/kg)和El组(200 mg/kg)。

英文摘要:

      In this study we investigated the effects of vitamin E on the reproduction and the egg quality of tongue sole Cynoglossus semilaevis. Broodstock were fed for 10 weeks with diet groups Ec, El and Eh that were supplemented with 0, 200 and 1200 mg/kg vitamin E (DL-α-tocopherol acetate) respectively. The results showed that subjects in the Eh group (1200 mg/kg Vitamin E) displayed advantageous development of gonad, and improvement in the relative fecundity, buoyant egg ratio, fertilization rate, hatching rate ratio and larvae length, as well as higher quality of larvae. The larvae survival rate, larvae length at 12 days post hatching and survival activity index of larvae (SAI) of the Eh group were higher than those in the Ec group (P<0.05). The development of gonad and the larvae length in the El group (200 mg/kg vitamin E) were promoted, however the fecundity, buoyant egg ratio, fertilization rate and hatching rate ratio were not changed compared to those in the Ec group (P>0.05). Along with the increase in dietary vitamin E, the activity of superoxide ismutase (SOD) in serum was significantly increased and the level of malonaldehyde (MDA) was reduced (P<0.05). The levels of dry matter and vitamin C in eggs were significantly higher in the El and Eh groups (vitamin E supplementation groups) than in the Ec group (P<0.05), but there was no difference between the El and Eh groups. Furthermore, the addition of vitamin E in the diet also increased the levels of PUFA, n-3PUFA, n-3HUFA and n-6PUFA in eggs and thus improved the egg quality. These results demonstrated that vitamin E supplemented in the diet could promote the development of gonad and improve the fecundity, the quality of eggs and larvae of tongue sole, and that 1200 mg Vitamin E per kg was optimal for the reproductive performance of tongue sole.