吸附法制备大麻哈鱼硫酸软骨素的工艺优化

Optimized absorption method to isolate chondroitin sulfate from Oncorhynchus keta

中文关键词: 大麻哈鱼  硫酸软骨素  分离纯化  树脂

英文关键词: Oncorhynchus keta  Chondroitin sulfate  Separation and purification  Resin  Adsorption

中文摘要:

      以去肉后的大麻哈鱼鱼头为原料，采用复合酶酶解提取硫酸软骨素，研究D958树脂分离纯化硫酸软骨素的效果；以解吸率为指标，研究吸附温度、时间及酶解液pH对硫酸软骨素纯化效果的影响。结果表明，采用D958树脂纯化大麻哈鱼鱼头硫酸软骨素的最佳工艺条件为: 酶解液pH 6.0，4℃吸附3 h；此条件下硫酸软骨素的解吸率达87.66%，纯化效果最好。因此树脂吸附法能从大麻哈鱼鱼头酶解液中有效分离得到硫酸软骨素，为大麻哈鱼加工废弃物的高值化利用开辟了一个新途径。

英文摘要:

      The levels of chondroitin sulfate in Oncorhynchus keta are low, and the traditional method to separate and purify chondroitin sulfate primarily focus on cartilage. The present study was conducted to establish a new method to effectively obtain chondroitin sulfate from the low content raw materials. Chondroitin sulfate was prepared and isolated from the fish head through compound protease digestion and D958 resin adsorption. We also evaluated the effects of pH value of enzymatic hydrolysate, adsorption temperature and time on purification using the desorption capacity of chondroitin sulfate as an index. The results showed that the optimum purification conditions using D958 resin were as follows: the pH of enzymatic hydrolysate was 6.0, and the absorption temperature and time were 4℃ and 3 h respectively. The desorption rate was up to 87.66% under the optimum conditions. Chondroitin sulfate was effectively purified by the resin adsorption method. We have developed a key method to separate and purify chondroitin sulfate from low content raw material, which pave a new pathway for the utilization of wastes from O. keta processing.