单过硫酸氢钾粉对5种水产常见致病菌的体内外抑菌作用

Bacteriostasis in vitro and in vivo of potassium monopersulfate powder on five strains of common pathogenic bacteria in aquaculture

中文关键词: 单过硫酸氢钾  水产致病菌  抑菌作用

英文关键词: Potassium monopersulfate  Pathogenic bacteria in aquaculture  Bacteriostatic activity

中文摘要:

      研究了单过硫酸氢钾粉对5种水产常见致病菌的体内、外抑菌作用，测定了体外最小抑菌浓度（MIC）、最小杀菌浓度（MBC）及以保护率为指标的体内抑菌效果。结果表明，体外抑菌实验中，单过硫酸氢钾粉对爱德华氏菌Edwardsiella、温和气单胞菌Aeromonas sobria、嗜水气单胞菌Aeromonas hydrophila、迟钝爱德华氏菌Edwardsiella tard、荧光假单胞菌Psudomonas fluorescens均有明显的抑制作用，其对5种菌的MIC分别为1、0.25、0.125、0.25、0.5 μg/ml；MBC与MIC相同。而体内抑菌实验中，随着单过硫酸氢钾粉浓度的增加，由5种致病菌引起鲫鱼的死亡率逐步降低。统计分析发现，使用0.25 μg/ml单过硫酸氢钾粉药浴鲫鱼，即可对由5种致病菌引起的鲫鱼病害有治疗的作用。

英文摘要:

      The bacteriostatic activity of potassium monopersulfate powder on five strains of common pathogenic bacteria in aquaculture was studied, and the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) in vitro and protection rate in vivo were determined respectively. Results of in vitro bacteriostatic experiments showed that Edwardsiella, Aeromonas sobria, A. hydrophila, Edwardsiella tard, and Psudomonas fluorescens were significantly inhibited by potassium monopersulfate powder. The MIC for these bacteria were 1, 0.25，0.125，0.25 and 0.5 μg/ml respectively, and the MBC were the same as the MIC. While for in vivo experiments, with the increase of concentration of potassium monopersulfate powder, mortality of Carassius auratus caused by the five pathogens decreased gradually. Statistical analysis showed that dipping C. auratus in 0.25 μg/ml potassium monopersulfate was effective in treating disease caused by the five pathogens in C. auratus.