高效液相色谱法测定卤虫无节幼体中精胺含量

Determination of spermine in brine shrimp by HPLC

中文关键词: 精胺  卤虫  高效液相色谱

英文关键词: Spermine  Brine shrimp  HPLC

中文摘要:

      本研究建立了高效液相色谱法测定卤虫无节幼体中精胺含量的方法。样品经5%三氯乙酸溶液提取，提取液采用丹璜酰氯柱前衍生，以甲醇-水为流动相，经C18反相色谱柱分离后用紫外检测器检测。精胺的线性范围为0.1~103μg/ml，线性相关系数0.9999，检出限为0.06μg/ml。3个添加浓度(0.1、1.0和5.0μg/ml)的平均回收率范围为86.3%~96.3%，相对标准偏差皆小于5%。采用此方法对卤虫中精胺进行检测，其精胺含量为0.077mg/g。本方法具有分析时间短，线性范围宽，灵敏度和精密度高等优点，适合于卤虫中精胺的检测。

英文摘要:

      An analytical method was established for the determination of spermine in brine shrimp by high performance liquid chromatography (HPLC) with ultraviolet detector. Spermine in brine shrimp samples was extracted with a mixture of 5% TCA, then derived with dansyl chloride, followed by separation via the C18 RP-HPLC column with methanol-water. Under the optimal conditions, spermine showed good linear relationship in the range of 0.1~103μg/ml and the correlation coefficient (R2) was 0.9999. At the spiked levels of 0.1, 1.0 and 5.0μg/ml, the average recoveries were 86.3%~96.3% with the relative standard deviations less than 5%. The minimum detection concentration was 0.06μg/ml. The overall process was successfully applied to identify and quantify spermine in brine shrimp, and the value was 0.077mg/g. The method is sensitive, rapid and precise.