鳗鲡外周血白细胞体外吞噬嗜水气单胞菌数量模型的建立

Establishment of quantitative model on eel peripheral blood leukocytes phagocytosing Aeromonas hydrophila in vitro

中文关键词: 外周血白细胞  吞噬  嗜水气单胞菌  鳗鲡

英文关键词: Peripheral blood leukocyte  Phagocytosis  Aeromonas hydrophila  Eel

中文摘要:

      为建立鳗鲡外周血白细胞体外吞噬嗜水气单胞菌的数量模型，本文研究了感染时间一定感染复数变化的情况下鳗鲡外周血白细胞吞噬的细菌数量和白细胞的存活率，同时还研究了感染复数一定感染时间变化的情况下鳗鲡外周血白细胞吞噬的细菌数量和白细胞的存活率。结果发现，鳗鲡外周血白细胞对嗜水气单胞菌有较强的吞噬作用，在感染复数为100:1、感染时间为30min时细胞吞噬的细菌量达到饱和，而细胞本身仍可维持较高活性，表明100:1的感染复数及30min的感染时间是研究鳗鲡外周血白细体外吞噬嗜水气单胞菌数量模型的最佳参数。本文还通过检测吞噬速率和杀菌速率评估建立起来的数量模型，研究结果发现，鳗鲡外周血白细胞吞噬嗜水气单胞菌的速率较吞噬大肠杆菌的速率快，但杀灭该菌的速率却均显著低于杀灭大肠杆菌和金黄色葡萄球菌的速率，进一步证明了建立的数量模型具有良好的可行性。

英文摘要:

      In order to establish the quantitative model on eel peripheral blood leukocytes phagocytosing Aeromonas hydrophila in vitro, we first examined the variety of the number of bacteria phagocytosed by eel peripheral blood leukocytes and the survival ratio of the leukocytes when the infection time was unvariable and infection multiplicity was changed, as well as when infection multiplicity was stable and infection time changed. The results showed that the phagocytosis of eel peripheral blood leukocytes to A. hydrophila was significant, when the infection multiplicity was 100:1 and the infection time was 30 min, the number of the bacteria that phagocytosed by eel peripheral blood leukocytes reached saturation while the leukocytes still maintained high activity, indicating that 100:1 and 30 min was the optimal infection multiplicity and infection time. In addition, the quantity model was validated by detecting predation velocity and sterilization velocity. It was found that the phagocytosis velocity of eel peripheral blood leukocytes for A. hydrophila was faster than that for Escherichia coli, while the velocity for killing A. hydrophila was significantly lower than that for killing E.coli and Staphylococcus aureus, which shows that the quantity model established can be used for further study on intracellular survival mechanisms of A. hydrophila.