5个红鳍东方鲀养殖群体微卫星DNA遗传多态性分析

Polymorphisms analysis of five populations of Takifugu rubripes with microsatellite

中文关键词: 红鳍东方鲀  养殖群体  微卫星遗传  多态性

英文关键词: Takifugu rubripes  Cultured stocks  Microsatellites  Genetic polymorphisms

中文摘要:

      对中国国内主要养殖区域红鳍东方鲀种质资源情况进行调查，采用微卫星标记分析技术，选用20对微卫星引物，对国内来自不同养殖区的5个红鳍东方鲀养殖群体：文登群体（WD）、莱州群体（LZ）、日本群体1（J1）、日本群体2（J2）、唐海群体（THQ），进行遗传多样性分析。实验结果显示，12对引物具有多态性。5个群体的等位基因数为2~8个不等；平均观测杂合度分别为0.27、0.26、0.18、0.25、0.20；平均期望杂合度分别为0.55、0.57、0.69、0.71、0.62；平均多态信息含量排列顺序为J2（0.65）＞J1（0.63）＞THQ（0.55）＞LZ（0.51）＞WD（0.48）。分析结果表明，1）WD和LZ群体间遗传距离最小，为0.233 6；LZ与J1群体间遗传距离大，为0.624 2;2）WD群体遗传多样性为中度多态（0.25＜PIC＜0.5），LZ、J1、J2以及THQ群体遗传多样性均为高度多态（PIC≥0.5）。分析结果表明目前国内主要养殖区域红鳍东方鲀具有较高的遗传多样性，有较好的遗传改良潜力。

英文摘要:

      In order to determine the genetic diversity of Takifugu rubripes at five main culture areas in China, twenty pairs of microsatellite primers were tested on populations from Wendeng (WD), Laizhou (LZ), Japanese 1(J1), Japanese 2 (J2) and Tanghai (THQ). It was found that 12 pairs (60%) of the primers displayed polymorphism. The number of alleles of the five populations ranged from 2 to 8, and the average observed heterozygosity was 0.27, 0.26, 0.18, 0.25, 0.20, respectively; the average expected heterozygosity was 0.55, 0.57, 0.69, 0.71, 0.62, respectively; the order of polymorphism information content (PIC) was J2(0.65)＞J1(0.63)＞THQ(0.55)＞LZ(0.51)＞WD(0.48). The results showed that: (1) genetic distance between WD and LZ was 0.233 6, which was the lowest; the genetic distance between LZ and J1 was 0.624 2, which was the highest; (2) the genetic diversity of WD was moderate (0.25＜PIC＜0.5), while that of LZ, J1, J2 and THQ were relatively high (PIC≥0.5). The analysis showed that the genetic diversity of T. rubripes at China’s main culture regions was very high, and therefore, the populations had relatively high potential for genetic improvement.