微胶囊剂型黄芪多糖对刺参生长性能、免疫力及抗病力的影响

Impact of APS microcapsules on growth,immunity and disease resistance of sea cucumber Apostichopus japonicus

中文关键词: 微胶囊  黄芪多糖刺参  生长  免疫  抗病力

英文关键词: Microcapsule  Astragalus polysaccharide  Apostichopus japonicas  Growth  Immune  Disease resistance

中文摘要:

      在基础饲料中添加不同剂量(1%、3%、5%)的微胶囊剂型黄芪多糖(Astragalus polysaccharudes,APS) (载药量为17%)，制成3种配合饲料，连续投喂初始体重为22.0±1.0g的刺参Apostichopus japonicus 35d，探讨微胶囊剂型黄芪多糖（以下简称“微胶囊”）对刺参生长性能、非特异性免疫及抗病力的影响。结果显示，35d后刺参最高特定生长率(Specific growth rate, SGR)为0.51%/d(5%添加组)(P<0.05)；微胶囊添加量为3%时免疫效果最佳，刺参体腔液中酸性磷酸酶ACP、超氧化物歧化酶SOD、髓过氧化物酶MPO活性显著高于对照组(P<0.05)。试验期间每隔7d测定刺参体腔液样品，其中ACP活性在第28天最高，为11.2U/100ml (P<0.05)；SOD活性在第21天最高，为63.3U/ml(P<0.05)；MPO活性在第21天最高，为40 MPO单位/ml(P>0.05)；免疫试验结束后对刺参进行灿烂弧菌Vibrio splendidus攻毒实验，14d内观察得出，3%微胶囊添加组累积死亡率明显低于对照组 (P<0.05)。由此可得，微胶囊剂型黄芪多糖对仿刺参生长性能、免疫力及抗病力方面均具有显著的增强效果，以3%添加量效果最佳，且添加量、作用时间、免疫效果之间存在相关性。

英文摘要:

      The study was conducted to investigate the impact of Astragalus polysaccgarudes(APS) microcapsules (drug loading 17%) on the growth, nonspecific immune indices and resistance of Apostichopus japonicas against Vibrio splendidus infection.APS microcapsules were added at graded levels (1%, 3%, and 5%) to the basal diet to formulate three practical compound diets. Continuous 35-day feeding trials were carried out on A. japonicus at initial body weight of 22.0±1.0g. After feeding the diets for 35 days, specific growth rate (SGR) for the 5% treatment was 0.51%/d, which was significantly higher than the control. The activities of serum acid phosphatase (ACP), superoxide dismutase (SOD), and myeloperoxidase (MPO) increased significantly (P<0.05) in the 3% treatment. During the experiment these factors were determined at 7-day intervals. The activity of ACP was 11.2U/100ml at 28d during the experiment (P<0.05), the activity of SOD was 63.3U/ml at 21d (P<0.05), and the activity of MPO was 40MPO unit/ml at 21d (P>0.05). After the feeding trial, a V. splendidus injection challenge was given to the sea cucumbers to test the effects of APS microcapsule. The 3% treatment showed significantly better survival than the control (P<0.05). It is suggested that supplementation of 3% APS microcapsules has the greatest effects on growth, immunity and disease resistance in A. japonicas against V.splendidus. In addition, there was a direct relationship between the level and time of treatment, and the immunity.