养殖刺参“化板症”病原菌的分离与鉴定

Isolation and identification of a pathogenic bacterium associated with off plate syndrome from Apostichopus japonicus cultured in Liaoning Province

中文关键词: 刺参  化板症  副溶血弧菌  16S rDNA  鉴定

英文关键词: Apostichopus japonicus  Off plate syndrome  Vibrio parahaemolyticus  16S rDNA  Identification

中文摘要:

      本研究对辽宁4家刺参育苗场患“化板症”的稚参进行了病原学分析，从患有“化板症”的稚参体表病灶处分离得到一株优势菌Aj2010072802A90。人工回接感染试验证实，该细菌能使稚参发生厌食、萎缩、降低附着力、溃烂、死亡等现象，具有较强的致病性，为此次“化板病”的病原菌。在此基础上，利用细菌形态观察、生理生化及16S rDNA 分子生物学方法对该菌株进行了鉴定，结果显示，该菌为副溶血弧菌Vibrio parahaemolyticus。这是副溶血弧菌导致海参感染的首次报道。此外，本研究针对该病原菌进行了药敏学测试，证实所分离的副溶血弧菌菌株对氟苯尼考等药物高度敏感，相关研究结果将为刺参疾病防控和健康养殖提供了理论依据和技术支撑。

英文摘要:

      The off plate syndrome is an epidemic disease of juvenile sea cucumber Apostichopus japonicus during nursery period, showing severe infection, rapid transmission and high mortality. In this study, epidemiological investigation of off plate syndrome was conducted in several infected hatcheries along the coast of Liaoning Province. A dominant bacteria strain coded Aj2010072802A90 was isolated from the skin of affected juveniles and proved to be the pathogen through the artificial infection tests. The pathogen was identified as Vibrio parahaemolyticus through morphological observation, physiological & biochemical tests and 16S rDNA sequence analysis. This is the first report on V. parahaemolyticus disease in juvenile sea cucumber. Drug sensitive tests were done against the pathogen and revealed that the V. parahaemolyticus strain was sensitive to florfenicol. The result would provide theoretical basis and technical support for disease control and health management in sea cucumber culture.