鱼鳞胶原-壳聚糖止血海绵的制备工艺

Research on preparation technique of fish scale collagen-chitosan hemostatic sponge

中文关键词: 鱼鳞胶原  壳聚糖  止血海绵  交联

英文关键词: Fish scale collagen  Chitosan  Hemostatic sponge  Crosslinking

中文摘要:

      以罗非鱼Tilapia mossambica鱼鳞为原料，经低温酶解提取鱼鳞Ⅰ型胶原，将鱼鳞Ⅰ型胶原与壳聚糖进行混合交联，冻干后制成胶原 壳聚糖止血海绵。以止血海绵的密度、吸水倍数、保水率、透气率和止血时间为指标，通过单因素实验和正交试验优化获得最佳的制备工艺：胶原与壳聚糖配比7∶3、制备液总浓度1.4 %、交联剂添加量0.015 %。在该最佳工艺下制备的止血海绵密度为22.95 mg/cm3、吸水倍数为45.34、保水率为55.83%、透气率为46.36%、止血时间为68.0 s，呈蜂窝状多孔结构，具有优良的持水性和透气性。

英文摘要:

      Fish scale type I collagen was extracted through low temperature enzymolysis from Tilapia mossambica, and after being crosslinked with chitosan, hemostatic sponge was generated via freeze-drying. Through single factor comparative tests and orthogonal tests, the optimum preparation technique was obtained as follows: mixing of collagen with chitosan according to a given volume ratio 7∶3, maintaining total concentration of preparation mixture at 1.4%, and keeping dosage of cross-linking agent at 0.015%. Under this condition, the generated hemostatic sponge possessed excellent properties, such as density (22.95 mg/cm3), water absorption (45.34), water retention (55.83%), breathability (46.36%), and hemostatic time (68.0 s). A faveolate vesicular structure, which contributed to preferable properties, was clearly observed with SEM.