不同强化物质对卤虫体内游离氨基酸含量的影响

Effects of different enrichment material on the free amino acid in Artemia nauplii

中文关键词: 赖氨酸  卤虫  游离氨基酸

英文关键词: Lysine  Artemia  FAA

中文摘要:

      以卤虫无节幼体作为试验材料，研究不同强化物质对卤虫体内游离氨基酸(FAA)含量的影响。试验分为4组，分别强化赖氨酸(Lys)、裂壶藻Schizochytrium、赖氨酸加裂壶藻以及空白对照组，试验进行16 h，每4h取样1次。结果显示，试验结束时，赖氨酸组的卤虫体内游离Lys含量显著高于其他3个组，且较强化前相比增加了4倍多，裂壶藻组的含量最低。除苏氨酸(Thr)外，赖氨酸组其他游离必需氨基酸以及天冬氨酸(Asp)、丝氨酸(Ser)、甘氨酸(Gly)、酪氨酸(Tyr) 含量都显著高于其他3个组。赖氨酸加裂壶藻组，Thr、Ser、Gly、丙氨酸(Ala)、组氨酸(His)和脯氨酸(Pro)含量均有不同程度的减少，其余组的卤虫，只有Thr含量减少，其他几种FAA水平均增加。试验过程中FAA水平随时间的变化趋势，空白组和赖氨酸加裂壶藻组相同，除Thr外，其余FAA含量都是在4 h时增高，8 h时下降，12 h时升高；而在赖氨酸组和裂壶藻组的卤虫体内，这些FAA含量是前8 h升高，12 h时降低，16 h时又升高。

英文摘要:

      Effects of different enrichment material on the free amino acid in Artemia nauplii was studied. There were 4 groups, including lysine group, Schizochytrium group, lysine plus Schizochytrium group and control group. The experiment lasted 16 hours, and was sampled every 4 hours. The results showed that the content of free lysine in Artemia nauplii in lysine group was significantly higher than the other groups and 4 times higher than 0h at the end of the experiment. And the content of lysine in Schizochytrium group was the lowest. Except Thr, the content of other free essential amino acids and Asp, Ser, Gly, Tyr in lysine group were significantly higher than other groups. The content of Thr, Ser, Gly, Ala, His and Pro of lysine plus Schizochytrium group decreased as the time prolonged. But in other groups, the content of FAA all increased except Thr. The variation trends of the FAA level in the control group was similar to the lysine plus Schizochytrium group: except Thr, the content of FAA increased at the first 4 h, declined at 8 h, and increased at 12 h. But in lysine group and Schizochytrium group, the content of these FAA in Artemia nauplii increased during the first 8 h, declined at 12 h,and then increased at 16 h.