南极磷虾酶解多肽的抑菌活性

Antimicrobial activity of polypeptides from Antarctic krill

中文关键词: 南极磷虾  多肽  抑菌活性

英文关键词: Antarctic krill  Polypeptide  Antimicrobial activity

中文摘要:

      选用复合酶酶解南极磷虾，对酶解多肽进行多肽含量及相对分子质量分布测定。采用滤纸片法筛选南极磷虾酶解多肽及各分级级分对金黄色葡萄球菌、枯草杆菌、四联微球菌、大肠杆菌、啤酒酵母和副溶血弧菌6种菌的抑菌活性。结果表明，该法所得的南极磷虾酶解多肽的含量达81.44%，相对分子质量范围为254~10 000；酶解多肽对金黄色葡萄球菌有明显的抑菌活性，且分级级分Ⅲ（1.0×103＜Mr＜3.0×103）的抑菌效果明显优于其他级分。

英文摘要:

      In the paper, combined enzymes were used for the hydrolysis of Antarctic krill Euphausia superba to obtain polypeptides, and the polypeptides contents and molecular weight distribution were determined by biuret method and liquid chromatography respectively. The antimicrobial activity of peptides and different molecular-weight fractions of polypeptides to six strains including Staphylococcus aureus, Bacillus subtilis, Micrococcus tetragenus, Escherichia coli, Saccharomyces cerevisiae, and Vibrio parahaemolyticus was carried out by the filter paper method. The results showed that polypeptides contents of the hydrolysate was 81.44% and the range of peptides molecular weight was 254~10 000. The polypeptides from Antarctic krill presented obvious antibacterial activity to S. aureus, and fraction Ⅲ（Mr＜3.0×103） showed the strongest antimicrobial activity.