鲟鱼硫酸软骨素的制备工艺研究

Study on extraction of chondroitin sulfate from Amur sturgeon

中文关键词: 史氏鲟  软骨  碱提  酶解  醇沉  硫酸软骨素

英文关键词: Amur sturgeon  Cartilage  Alkali extraction  Enzymatic hydrolysis  Alcohol precipitation  Chondroitin sulfate

中文摘要:

      以史氏鲟鱼软骨为原料，按碱提 酶解 乙醇沉淀工艺流程进行提取硫酸软骨素的各主要工序工艺的研究。通过单因素试验及正交试验，得出最佳碱提工艺为以两倍量6%的 NaOH为溶剂，于40 ℃的水浴中搅拌提取4 h。通过正交试验，得出最佳酶解工艺条件是先将浸提液调节pH至8.6，加入胰酶0.5%，50 ℃保温水解3 h；后降温至40℃，调pH至5.7，加入胃蛋白酶0.3%，保温水解2h。通过单因素试验，得出最佳的乙醇沉淀工艺条件是将滤液pH调为6.5，加入95%的乙醇至醇沉浓度为70%，静置过夜。制备的硫酸软骨素经检测符合WS1-C3-0030-2000（硫酸软骨素口服片）和WS-10001-(HD-0892)-2002(硫酸软骨素注射品)标准的要求。

英文摘要:

      The single factor test and the orthogonal test results showed that the optimum alkali extraction process was: 2 volumes of 6% NaOH were added, followed by stirring in water bath at 40℃ for 4h. The orthogonal test results indicated that the optimum enzymolysis procedure was as follows: 0.5% pancreatin was added to the extraction solution adjusted to pH 8.6, and kept at 50℃ for 3h for hydrolysis, and then 0.3% pepsin was added to the solution adjusted to pH 5.7 and kept at 40℃ for 2h. The single factors test results of ethanol solution revealed that the best procedure was as follows: after adjusting pH of the obtained hydrolysate to 6.5, 95% ethanol was added to adjust the consistency of precipitation system to 70%. The quality of the product met the standard requirement of WS1-C3-0030-2000 (Chondroitin sulfate tablets) and WS-10001-(HD-0892)-2002 (Chondroitin sulfate injection).