WSSV感染对克氏原螯虾血细胞吞噬和肝胰腺磷酸酶活性的影响

Impacts of WSSV on the activity of haemocyte phagocytosis and hepatopancreas phosphatase in Procambarus clarkia

中文关键词: WSSV  克氏原螯虾  吞噬活性  碱性磷酸酶  酸性磷酸酶

英文关键词: WSSV  Procambarus clarki  Phagocytosis  AKP  ACP

中文摘要:

      分析了克氏原螯虾Procambarus clarkia人工感染白斑综合征病毒(White Spot Syndrome Virus,WSSV)后，其血细胞吞噬(Phagocytosis)活性，肝胰腺酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性的变化规律，其目的是为WSSV感染与螯虾的免疫防御反应等研究提供依据。分析结果显示，随着WSSV感染克氏原螯虾时间的延长，血细胞吞噬活性、肝胰腺酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性也随之改变，其中血细胞吞噬活性的变化规律性比较明显，可作为WSSV感染的敏感指标，也可以用作间接指示克氏原螯虾健康状况的指标。而在不同的感染时间，克氏原螯虾肝胰腺ACP与AKP的活性波动较大，无明显的规律可循，难以作为WSSV感染的敏感指标。

英文摘要:

      The impacts of White Spot Syndrome Virus (WSSV) on the haemocyte phagocytic activity,and the acid phosphate (ACP) and alkaline phosphate (AKP) activities in the hepatopancreas of Procambarus clarkia were examined.The results showed that the immune factors of P.clarkia changed along with the WSSV infection time,and phagocytes activity could reflect the health of shrimps indirectly.However,the variation of the ACP and AKP activity was not obvious and had no apparent tendency during different infection stages.This study provided basic data on immune level change after pathogenic affection.