不同载体固定化海洋微生物酯酶ETM-b的性能比较

Study on immobilization of marine microbial esterase ETM-b with different material

中文关键词: 海洋芽孢杆菌酯酶  固定化酯酶  壳聚糖  非水介质催化

英文关键词: Esterase from Bacillus sp.  Immobilized esterase  ChitosanNon-aqueous catalysis

中文摘要:

      分别采用海藻酸钠包埋法、明胶包埋交联法、壳聚糖吸附交联法制备固定化海洋芽孢杆菌酯酶ETM-b，并对其固定化条件进行了研究。结果发现，壳聚糖制备的固定化酶效果最好，壳聚糖2%、戊二醛浓度1%、小球与酶液4∶3（g/ml）时制备的固定化酶的活性回收最高，达到66%。壳聚糖制备的固定化酶使用10次，相对活性保留70%，具有良好的操作稳定性。固定化酶在非水介质中具有转化α-乙酸萘酯(α-Naphthyl acetate)的能力，在异辛烷、正辛烷、正己烷中活性表现最高。

英文摘要:

      Esterase from Bacillus sp. was immobilized with sodium alginate, gelatin and chitosan. The enzyme, immobilized with 2% chitosan, 1% glutaraldehyde and chitosan microspheres∶enzyme loading=4∶3 (g/ml) exhibited the highest activity recovery (66%). The immobilized esterase with chitosan could be reused for 10 times, and 70% of enzyme activity remained after the 10th batch, which exhibited good operational stability. The immobilized esterase can catalyze α-naphthyl acetate in non-aqueous system, and it displayed high activity in isooctane, n-octane and n-hexane.