利用重楼杀灭罗氏沼虾寄生纤毛虫的初步研究

Study on efficacy of Rhizorma paridi against the ciliates on giant freshwater shrimp Mactobrachium rosenbergii

中文关键词: 重楼  纤毛虫  罗氏沼虾  活性成分

英文关键词: Rhizorma paridin  Ciliates  Giant freshwater shrimp  Active substance

中文摘要:

      利用系统溶剂极性法提取重楼，制备粗提物，以寄生罗氏沼虾上的纤毛虫（聚缩虫和靴纤虫）为指示寄生虫，进行杀灭罗氏沼虾寄生纤毛虫的药效活性追踪试验，初步确定重楼杀灭纤毛虫的活性部位，并对活性部位进行安全性评价。实验结果表明，不同极性的重楼提取物对纤毛虫均有一定的杀灭作用，其中重楼甲醇提取物对纤毛虫的杀灭作用最强，其浓度为45 mg/L时，对纤毛虫杀灭率为100%，确定其含有杀灭纤毛虫的活性成分。活性部位对罗氏沼虾仔虾急性毒性实验结果显示，甲醇提取液对仔虾的96 h半致死浓度（LC50）为272 mg/L，其安全浓度为111.5 mg/L，表明重楼是一种比较安全、杀虫效果良好的水产用中草药。

英文摘要:

      To screen out the active parasiticidal components, solvent extractions from Rhizorma paridis were evaluated against ciliates (Zoothamnium sp. and cothurnia) with batch treatment. The bioassay based isolation showed that methanol extract presented 100% antihelmintic efficacy at the concentration of 45 mg/L and the killing active site was proved to be its methanol extract. Meanwhile, the median lethal concentration (LC50) values at 96 h determined from the acute toxicity tests of the killing active site for juvenile giant freshwater shrimp was 272 mg/L. The safety concentration was 111.5 mg/L. The results obtained in this study show that Rhizorma paridis could be a potential plant based medicine for ciliates control.