刺参体腔液穿刺抽取后细胞恢复过程的初步研究

Coelomocyte resume of sea cucumber Apostichopus japonicus after puncturable extraction of the coelomic fluid

中文关键词: 刺参  体质量  体腔液  体腔细胞  穿刺  恢复

英文关键词: Sea cucumber  Apostichopus japonicus  Body weight  Coelomic fluid  Coelomocyte  Puncture  Resume

中文摘要:

      通过所绘制的刺参沥水后体质量变化曲线得出，在气温20 ℃以下，刺参沥水后20~30 min为测量刺参体质量的最佳时机。利用重量差值法得出刺参体腔液占体质量的百分比分别是：小参26.88±1.39%、中参25.24±2.91%、大参30.98±1.86%。通过检测刺参体腔液抽取后刺参生理状态的变化得出刺参体腔液单次安全抽取量为体质量的1.5%以内。通过检测单次抽取刺参体腔液后其体腔液中总细胞、透明细胞及淋巴样细胞和颗粒细胞数量的恢复过程得出，刺参不同类型的体腔细胞在受抽取刺激后恢复过程也不是同步进行的，体腔液总细胞浓度恢复时间间隔为4 d，颗粒细胞、透明细胞及淋巴样细胞浓度恢复时间间隔为6 d，体腔液吸光值恢复时间间隔为5 d，由此得出，在进行多次抽取体腔液时建议至少间隔6 d以上。研究建立的刺参体质量测量方法和所确定刺参体腔液基础指标为进一步开展刺参免疫学、生理学、动物学试验等研究提供参考和基础性数据。

英文摘要:

      The optimum measurement for sea cucumber Apostichopus japonicus body weight and coelomic fluid is an essential experiment for the related research. The results show that the body weight is suitable to weigh in 20～30 min after being taken away from culture water (below 20 ℃). The proportion of coelomic fluid to the total body weight was also examined for small, medium and large sized sea cucumbers, at 26.88±1.39%, 25.24±2.91% and 30.98±1.86%, respectively. The safe dose of coelomic fluid extraction was less 1.5% to the total body weight of sea cucumber for ensuring physiological stability and survival of the tested animals. The results illustrated that the total cells of sea cucumber experienced a process of significantly reducing to slowly recovering. The resume process was asynchronous for different kinds of coelomocyte. The results show that the resume period for total cells, granule cells, transparent lymphoid cells and the absorbency of coelomic fluid are 4 d, 6 d, 6 d and 5 d, respectively. The measurement of body weight, the proportion of coelomic fluid to the total body weight, the safe dose and the resume process of coelomic fluid is firstly reported in sea cucumber Apostichopus japonicus. This research could be helpful for further study on physiological, immunological and zoological research in sea cucumber..