大黄鱼Myostatin-1基因启动子克隆及生物信息学分析

Cloning and bioinformatic analysis of myostatin-1 gene promoter in Larimichthys crocea

中文关键词: 大黄鱼  Myostatin-1启动子  克隆  生物信息学分析

英文关键词: Larimichthys crocea  Myostatin-1 promoter  Cloning  Bioinformatic analysis

中文摘要:

      Myostatin基因属于TGF β超家族,是动物骨骼肌生长发育的负调控因子。在已克隆大黄鱼Myostatin-1基因编码序列的基础上，采用基因组步移的方法，首次克隆到约1k bp的大黄鱼Myostatin-1启动子序列。该启动子序列与已报道的金鲷和大口黑鲈相应序列的相似度分别为90%和75%。该序列具有1个TATA box、1个CAAT box、5个E-box位点，此外还具有MyoD，USF，MEF2，SP1，NF.-Y等多种转录因子结合位点

英文摘要:

      Myostatin is a member of the transforming growth factor-β superfamily, which functions as a negative regulator for the development and growth of skeletal muscle in animal. Based on the cloned myostatin-1 gene of Larimichthys crocea, the promoter sequence of L. crocea myostatin11 gene was cloned by genomic walking. The sequencing results indicate that it shares 90% and 75% homology identity with the corresponding reported sequences of Sparus aurata and Micropterus almoides, respectively. Bioinformatic analysis showed that myostatin-1 gene contains one consensus sequences of TATA box, a CAAT box and five putative E-boxes known as the binding sites to myogenic basic helix-loop helix transcription factors. In addition, it also possesses many transcription factor binding sites, such as MyoD, USF, MEF2, SP1 and NF-Y. These results are expected to be the basis for further studies on the regulation and expression of this gene.KEY WORDSLarimichthys croceaMyostatin-1 promoterCloningBioinformatic analysis