魁蚶精子的超微结构

Ultrastructure of the spermatozoon of Scapharca broughtonii

中文关键词: 魁蚶  精子  超微结构

英文关键词: Scapharca broughtonii  Spermatozoon  Ultrastructure

中文摘要:

      应用扫描和透射电镜观察了魁蚶的精子，揭示了其超微结构。扫描结果显示，魁蚶精子全长55 μm，由头部、中段和尾部3部分组成。其中，头部长约2μm，最宽处直径为1.5μm。透射结果显示，魁蚶头部包括顶体和精核。顶体位于头部前端，较圆钝，呈倒“V”形，与精核之间有一间隙，精核位于顶体的下方，占头部体积的2/3左右，具有核后窝和植入窝，但是没有核前窝。中段由5个近圆形的线粒体围绕着中心体构成，线粒体横切面长轴直径为0.6 μm，短轴直径为0.5 μm。尾部细长，由尾丝包被质膜构成，内有轴丝。横切面呈圆形，直径为0.15 μm，横切面为典型的“9+2”结构。

英文摘要:

      The ultrastructure of the spermatozoon of Scapharca broughtonii was observed with scan and transmission electron microscopy (SEM and TEM). The results of SEM demonstrated that the total length of spermatozoon was 55μm,composed of head,middle piece and tail.The head length of spermatozoon was 2 μm and the diameter was 1.5 μm at the maximum width.The results of TEM demonstrated that the head of spermatozoon consisted of acrosome and nuclear.The acrosome was in the front of head, somewhat obtuse and V-shaped upside down.A gap existed between the acrosome and the nuclear.The nuclear, in which there were posterior nuclear fossa and implantation fossa,without anterior nuclear fossa, was under acrosome and accounted for 2/3 of the head volume.The middle-piece was formed by centrosome which was revolved around by 5 oval-shaped mitochondrias.The long axis diameter of cross section at mitochondria was 0.6μm and the short axis diameter was 0.5μm. The flagellum was slender and consisted of tail fiber which was surrounded by plasma membrane,with an axoneme inside. The cross section was circular and its diameter was 0.15 μm, showing a typical “9+2” structure.