κ-卡拉胶寡糖硫酸基含量与其抗疱疹病毒活性的关系

Study on the relationship between the content of sulfate group and antiherpesviral activities of oligosaccharide from kappa-carrageenan

中文关键词: 卡拉胶寡糖  酶解  磺化  脱硫酸基  单纯疱疹病毒

英文关键词: κ-Carrageenan oligosaccharide  Enzymatic hydrolysis  Sulfating  Desulfating  HSV-1

中文摘要:

      利用酶解法降解κ-卡拉胶，得到κ-卡拉胶寡糖（KOS）。利用甲酰胺-氯磺酸和二甲基亚砜-甲醇-吡啶方法，分别得到κ-卡拉胶寡糖磺化衍生物（SKO）和κ-卡拉胶寡糖脱硫酸基衍生物（DSK）。利用单纯疱疹病毒HSV-1研究了κ-卡拉胶寡糖硫酸基含量与抗病毒活性之间的关系，同时从对病毒的直接作用、阻止病毒对细胞的吸附和影响病毒复制与释放3个途径初步探讨了κ卡拉胶寡糖抗疱疹病毒的机理。结果表明，KOS及SKO对Vero细胞毒性极低，对单纯疱疹病毒HSV-1无直接灭活作用，也不影响病毒的复制和释放,但是能够阻止病毒对细胞的吸附。相同浓度下，SKO对病毒吸附的抑制率要明显高于KOS，DSK对病毒则无明显作用。实验说明，κ-卡拉胶寡糖硫酸基含量与其抗疱疹病毒活性二者呈正相关，而且κ-卡拉胶寡糖及其磺化衍生物的抗病毒活性主要是通过阻止病毒对细胞的吸附而实现。

英文摘要:

      Carrageenan oligosaccharide (KOS) was prepared through enzymatic hydrolysis. After chemical modification of the produced oligosaccharide via use of formamide-chlorosulfonic acid or DMSO-methanol-pyridine, sulfated derivatives (SKO) and desulfated (DSK) derivatives of κ-carrageenan oligosaccharides were obtained respectively. Herpes simply virus (HSV-1) was used to study the relationship between the content of sulfate group and antiherpesviral activities of the oligosaccharide and its derivatives. The antiherpesviral mechanism of κ-carrageenan oligosaccharide was also studied by the ways of directly inactivating virus, inhibiting virus absorption and influencing virus replication and release. The results showed that KOS and SKO had little cytotoxic effect on Vero cells, and could not directly inactivate HSV-1 or influence its replication and release, but absorption of HSV-1 on the surface of cells was inhibited. SKO had stronger effects on HSV-1 absorption than KOS with the same concentration, while DSK had no effect on HSV-1. So it could be concluded that there was a positive relationship between the content of the sulfate group of κ-carrageenan oligosaccharide and its antiherpesviral activity, which was achieved mainly via inhibiting HSV-1 absorption on the host cells.