PolyI∶C不同途径诱导大菱鲆Mx蛋白基因的转 年际变化趋势及综合评价

Transcription of turbot Scophthalmus maximus Mx gene induced by polyinosinic polycytidylic acid given in different ways

中文关键词: PolyI∶C  大菱鲆  Mx蛋白  干扰素诱导剂  基因转录

英文关键词: Polyinosinic-polycytidylic acid (PolyI∶C)  Scophthalmus maximus  Mx protein  Interferon inducer  Gene transcription

中文摘要:

      以PolyI∶C（又称聚肌胞）为诱导剂，分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆 Scophthalmus maximus体内抗病毒蛋白Mx基因的转录，利用半定量RT-PCR方法检测干扰素下游基因-Mx基因的转录水平来确定该诱导剂的诱导效果。结果显示，以上3种途径都能高效诱导Mx蛋白mRNA的转录，均在48h之后达到高峰，其中以浸泡的方式更容易诱导Mx基因转录，且在120h时仍保持较高水平。Mx基因转录的时相变化证明了国产PolyI∶C可以通过多种途径诱导抗病毒蛋白Mx的表达，为实际应用中确定用药途径提供了理论依据。另外，实验初步建立了半定量RT-PCR方法，为检测鱼体内干扰素的表达提供了技术方法。

英文摘要:

      Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI∶C with intraperitoneal injection, immersion and oral administration. Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI∶C’s induction. The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI∶C application. The immersion method seemed to be the most effective way to induce Mx mRNA, as Mx mRNA could last more than 120 h at a high level. This experiment proved that the homemade PolyI∶C could effectively induce Mx mRNA transcription in different applications, which provided a reference to use PolyI∶C in aquaculture practice.