低温下大菱鲆有水和无水保活过程中生理生化变化的研究

Physiological and biochemical change of Scophthalmus maximuskept alive at low temperature with or without water

中文关键词: 大菱鲆  低温保活  生理生化

英文关键词: Scophthalmus maximus  Keeping alive at low temperature  Physiological and biochemical

中文摘要:

      研究了3±0.1℃保活条件下大菱鲆有水和无水状态下的成活率和生理生化指标的变化。结果表明，（1）大菱鲆有水保活72h成活率达到100%，无水保活60 h成活率为95%。（2）保活过程中大菱鲆肌肉中的ATP含量明显下降，而且无水保活的大菱鲆肌肉中ATP含量低于有水保活的。（3）保活过程中，大菱鲆血液指标MCV、BUN、CR和UA有随保活时间增加而升高的趋势，血液指标GLU、CHE有随时间增加而下降的趋势。有水保活的大菱鲆血液中BUN、CR、UA和GLU显著低于无水保活。通过对大菱鲆血液的生理生化指标分析，保活过程中大菱鲆死亡的原因之一是代谢产物不能有效排出，体内氨氮类有害物质含量过高。

英文摘要:

      In this experiment, the survival rate and change of physiological and biochemical indices of turbot Scophthalmus maximus which were kept alive at 3±0.1℃ were examined. The results showed that: ⑴ The survival rate after 60h was 95% without water. ⑵ The ATP contents of the muscle decrease obviously, and it was lower for fish kept in water than fish kept without water. ⑶ During the experiment, the MCV, BUN, CR and UA of the fish blood showed a trend of increase; however, the CR, UA and GLU for fish kept in water were obviously lower than those kept without water. The physiological and biochemical analysis revealed that, one of the reasons for fish mortality may be the inhibited excretion of metabolites, resulting in excessively high content of ammonia nitrogen which was harmful to the fish.