蛋白质与饱食度对工厂化养殖半滑舌鳎

Effects of protein and satiation degrees on growth and immunity of Cynoglossus semilaevis Günther in industrial culture

中文关键词: 半滑舌鳎  蛋白水平  饱食度  生长性能  免疫力

英文关键词: Cynoglossus semilaevis Günther  Protein levels  Satiation degree  Growth performance  Immunity activity

中文摘要:

      在封闭循环水养殖条件下，进行5×3双因素随机设计动物试验，即5种饲料蛋白质水平（43%、46%、49%、52%和56%；以A—E组表示）和3种饲喂饱食度水平（100%、90%和80%；以Ⅰ、Ⅱ和Ⅲ水平表示），共15个处理，每处理3重复，试验期108 d。探寻蛋白质营养与饱食度对工厂化养殖半滑舌鳎幼鱼（110±25 g）生长与免疫的影响。结果表明,（1）高蛋白水平与高饱食度投喂的增重效果最佳，E组增重率极显著高于其他组13.75%-50.16%,Ⅰ水平比Ⅱ和Ⅲ水平分别极显著提高7.57%和14.08%；中蛋白水平蛋白效率较高而死亡率最低，C组死亡率比其他组降低50%-75%；低饱食度的饲料利用率和蛋白质效率最高，Ⅲ水平比Ⅰ、Ⅱ水平分别显著高4.78%和5.32%。（2）中蛋白水平和高饱食度有利于提高超氧化物歧化酶（SOD）活力，C组比其他组显著提高4.2%-34.79%，Ⅰ水平分别比Ⅱ和Ⅲ水平极显著高15.27%和25.0%；中蛋白水平和90%饱食度有利于提高溶菌酶（LZM）活力，C组比其他组极显著提高4.61%-18.07%，Ⅱ水平比Ⅰ和Ⅲ水平分别极显著高12.03%和4.58%；中高蛋白水平和高饱食度有利于提高补体C3和C4活力。（3）获得工厂化养殖半滑舌鳎幼鱼最大生长的饲料蛋白质水平为56%；最佳免疫力和蛋白效率的饲料蛋白质水平为49%～52%。

英文摘要:

      he 5×3 two factor random animal experiment was conducted for 108 days to determine the effects of protein levels and satiation degrees on growth performance and immunity activity of juvenile Cynoglossus semilaevis Günther (with average initial weight of 110±25g) in the industrial culture condition with closed recirculation seawater. The trial fish were randomly allocated into 15 triplicated treatments,with five diets at protein levels of 43%，46%，49%，52%，56%(A E groups）and three satiation degrees of feeding（100%，90% and 80%, asⅠ和Ⅱ and Ⅲdegree）. The results indicated that: (1)the best weight gain were found in both the highest protein level and the highest satiation degree. Weight gain ratio in group E was significantly higher than the other groups (13.75%-50.16%),and that of degree Ⅰ was significant higher than degree Ⅱand Ⅲ (7.57% and 14.08%), respectively; higher protein efficiency ratio and the minimum mortality appeared in the intermediate protein level , mortality ratio of group C was lower than the others (50%-75%) the highest feed conversion ratio and protein efficiency ratio were present in the lowest satiation degree,for which degree Ⅲ was higher than the others (4.78% and 5.32%), respectively .(2) the intermediate protein level and the highest satiation degree were stimulative to enhancing SOD activity, group C was significantly higher than the other groups (4.2%-34.79%) degreeⅠwas significant higher than degree Ⅱ and Ⅲ (15.27% and 25.70%),respectively; the intermediate protein level and 90% satiation were good for promoting LZM activity, group C was significant higher than the other groups (4.61%-18.07%), degree Ⅱ was significant higher than degree Ⅰ and Ⅲ (12.03% and 4.58%), respectively; the intermediate and higher protein level and the highest satiation degree were stimulative to enhancing complement C3，C4 activity; (3) the protein level of feed for maximum growth of juvenile C. semilaevis Günther in the industrial culture condition was 56%, but for optimal immunity activity and protein efficiency ratio was 49%-52%.