产酯酶海洋微生物的筛选、鉴定及系统发育分析

Screening，identification and phylogenetic analysis ofmarine microorganisms producing extracellular esterase

中文关键词: 海洋微生物  酯酶  6S rDNA  系统发育学分析

英文关键词: Marine microorganism  Esterase  α Naphthyl acetate  Phylogenetic analysis

中文摘要:

      通过以三丁酸甘油酯和 α 乙酸萘酯为底物，建立了一种快速、准确的酯酶产生菌的筛选方法，并从样品中分离到 1 株产酯酶活力和稳定性较高的菌株 EB 1，对其进行了形态学、生理生化特征鉴定和 16S rDNA 序列分析。结果表明，该菌株与Bacillus subtilis subsp. subtilis 模式菌株的同源性高达 9979%；又根据其产黑色素这一特性，最终将其确定为Bacillus subtilis var. niger，其 16S rDNA 序列在 GenBank 的注册号为 EU016526。

英文摘要:

      With α naphthyl acetate and tributyrin as substrate，a precise and rapid screening method was developed．One stable strain EB 1，which was isolated from related samples，showed the highest esterase producing ability among the 281 strains．Through study on its morphological，physiological and biochemical characteristics as well as 16S rDNA sequence homology comparison，strain-EB-1 was identified as Bacillus subtilis var.niger，which was almost the closest relative to the standard strain with 9979% similarity in sequence under the phylogenetic tree．The 16S rDNA sequence of strain EB 1 has been deposited in GenBank，registration number was EU016526．