漠斑牙鲆引进群体子一代遗传多样性的RAPD分析

RAPD analysis of genetic diversity in first generation of introduced Paralichthys lethostigma

中文关键词: 漠斑牙鲆  遗传多样性  随机扩增多态DNA

英文关键词: Paralichthys lethostigma  Genetic diversity  RAPD

中文摘要:

      运用随机扩增多态性DNA（RAPD）技术对引进群体漠斑牙鲆Paralichthys lethostigma子一代48个个体的遗传多样性进行了分析，筛选的40个10 bp的随机引物中，25个引物产生了稳定性好，可重复性强的谱带。共检测到154个位点，其中多态位点有122个，多态位点的比例为79.22 %，有效等位基因数为1.562 9±0.363 1，Nei’s基因多样性指数为0.318 4±0.184 3，Shannon信息指数是0.465 1±0.257 4。结果表明，漠斑牙鲆处于较高的遗传多样性水平。

英文摘要:

      RAPD technique was used to analyze the genetic diversity of forty eight fish samples from the first generation of introduced Paralichthys lethostigma. Twenty five primers screened from 40 10bp primers produced highly repetitive and steady bands. 154 DNA fragments were identified, of which 79.22% (122 fragments) were polymorphic. The mean effective number of alleles per locus was 1.562 9±0.363 1,Nei’s gene diversity index was 0.318 4±0.184 3, and Shannon's information index was 0.465 1±0.257 4. All the results indicated that P. lethostigma had high genetic variability.