| 摘要: |
| 本研究以海南南渡江野生唇䱻(Hemibarbus labeo)为材料，采用腹腔注射植物血球凝集素(PHA)、秋水仙素，肾细胞直接制片法，分析唇䱻染色体核型和银染(Ag-NORs)。结果显示，唇䱻的二倍体染色体数目为50，核型公式为2n=18m+12sm+12st+8t，其染色体臂数(NF)为80，未发现异型染色体，Ag-NORs出现在第5对和第11对同源染色体的短臂末端。鮈亚科鱼类染色体进化综合分析结果表明，唇䱻具有鮈亚科鱼类的原始核型。本研究不仅补充了唇䱻的细胞遗传学数据，为研究鮈亚科/䱻属鱼类的分类与进化提供了数据资料，也为唇䱻的种质资源保护、种质改良等工作奠定了理论基础。 |
| 关键词: 唇䱻 染色体 核型 银染 鮈亚科 |
| DOI：10.19663/j.issn2095-9869.20190123002 |
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| 基金项目: |
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| The Karyotype and Ag-NORs of Hemibarbus labeo from Nandujiang River of Hainan |
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LI An1, LI Gaojun2, WANG Shifeng1, LIU Shufang3, ZHOU Yongcan1, SHEN Zhixin2, XUE Xianqiu1, CAI Yan1
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1.Ocean College, Hainan University, Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province, Haikou 570228;2.Hainan Academy of Ocean and Fisheries Sciences, Haikou 570100;3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071
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| Abstract: |
| In this study, the karyotype and Ag-NORs of wild Hemibarbus labeo from Nandujiang River of Hainan were analyzed using the methods of intraperitoneal injection of phytohemagglutinin (PHA) and colchicine, and direct preparation of head kidney cells. Results showed that H. labeo had a diploid chromosome number of 50, the karyotype formula was 2n = 18m + 12sm + 12st + 8t, the number of chromosome arms (NF) was 80, no heterotypic chromosome was observed, and Ag-NORs were located on the ends of the short arms of pair No.5 and pair No.11. Comprehensive analyses of the chromosomal evolution in subfamily Gobioninae indicated that H. labeo had the primitive karyotype of Gobioninae fish. Our study will not only supplement the cytogenetic data of H. labeo and provide information on the taxonomy and evolution of the subfamily Gobioninae, genus Hemibarbus, but will also lay the theoretical foundation for germplasm protection and germplasm improvement of H. labeo. |
| Key words: Hemibarbus labeo Chromosome Karyotype Ag-NORs Gobioninae |
