姜黄素对黄颡鱼(Pelteobagrus fulvidraco)生长、消化与抗氧化能力的影响

The Effects of Curcumin on the Growth, Digestion and Antioxidant Ability of Yellow Catfish (Pelteobagrus fulvidraco)

关键词:  姜黄素  黄颡鱼  生长  消化能力  抗氧化能力

Key words:  Curcumin  Pelteobagrus fulvidraco  Growth  Digestive activities  Antioxidant ability

摘要:

选取初始体重为(13.17±0.68) g、初始体长为(11.86±0.53) cm黄颡鱼(Pelteobagrus fulvidraco) 720尾，随机分为6组，每组3个重复，每个重复40尾鱼。分别投喂添加0、50、100、200、400、800 mg/kg姜黄素的实验饲料，分别标记为T1–T6。实验周期为60 d，实验结束后，测定黄颡鱼生长、消化和抗氧化能力指标。结果显示，各实验组特定增长率和存活率均显著高于对照组(P<0.05)，T4组增重率和蛋白质效率显著高于对照组(P<0.05)，T3、T4组饲料系数显著低于对照组(T1组) (P<0.05)；T3、T4、T5组前肠脂肪酶活力显著高于对照组(P<0.05)，T4组前肠淀粉酶活力显著高于对照组(P<0.05)；T6组肝胰脏、T5组脑与T4组头肾超氧化物歧化酶(SOD)活力均较对照组有显著提升(P<0.05)；脑实验各组丙二醛(MDA)含量均显著低于对照组(P<0.05)；T6组肝胰脏、T5组脾脏过氧化氢酶(CAT)活力显著高于对照组(P<0.05)；T4组肝胰脏、T5组脾脏、T5组中肾谷胱甘肽过氧化物酶(GSH-PX)活力显著提升(P<0.05)；血清实验各组GSH-PX活力均显著高于对照组(P<0.05)；肝胰脏实验各组谷胱甘肽(GSH)含量均显著高于对照组(P<0.05)；T5组肝胰脏、T4组脾脏和血清一氧化氮(NO)含量显著高于对照组(P<0.05)。研究表明，在饲料中添加适量的姜黄素可以显著提高黄颡鱼的生长性能、消化能力以及抗氧化能力，以200 mg/kg添加量为最佳。

Abstract:

A feeding trial was conducted to investigate the effects of dietary curcumin at different levels on the growth performance, digestive enzyme activities and antioxidant ability of yellow catfish (Pelteobagrus fulvidraco). Juvenile yellow catfish with an initial body weight of (13.17±0.68) g were fed with 6 different experimental diets containing curcumin at the concentration of 0 (control), 50, 100, 200, 400, and 800 mg/kg (namely T1, T2, T3, T4, T5 and T6 respectively) for 60 days. There were 3 replicates per group of 40 yellow catfish. The results were described as follows. The specific growth rate (SGR) and the survival rate (SR) of fish fed in the control group were significantly lower than those of all treatment groups (P<0.05), and the feed conversion ratio (FCR) was higher in T3 and T4 than the control group (P<0.05). The lipase activity in the foregut of fish from T3, T4 and T5 was significantly higher than the control group (P<0.05). The amylase activity in the foregut of fish from T4 was significantly higher than the control group (P<0.05). The level of superoxide dismutase (SOD) was higher in the hepatopancreas of fish from T6, in the brain of fish from T5 and in the head kidney of fish from T4 (P<0.05). A significantly lower content of malondialdehyde (MDA) was observed in the brain of fish from all treatment groups (P<0.05). In T5 and T6, there was a significant increase in the catalase (CAT) activity in the spleen and hepatopancreas (P<0.05). The glutathione peroxidase (GSH-PX) activity was enhanced (P<0.05) in the fish from T4, in the spleen of fish from T5 and in the mid-kidney of fish from T5, furthermore, it was also higher in the serum of fish from all treatment groups compared to the control group (P<0.05). The GSH activity in the hepatopancreas of fish from all treatment groups was higher than that in the control group (P<0.05). A significantly higher level of nitric oxide was found in the hepatopancreas of fish from T5 and in the spleen and serum of fish from T4, compared to all other treatment groups (P<0.05). The results indicated that 200 mg/kg curcumin supplemented into the diets could effectively boost the growth performance, and enhance the intestinal digestive enzyme activities and the antioxidant ability of juvenile yellow catfish.