制备方法对琼脂糖得率和质量的影响

Effect of processing method on the agarose productivity and quality

关键词:  琼脂糖  制备方法  得率  质量

Key words:  Agarose  Producing method  Productivity Quality

摘要:

研究了聚乙二醇法、硫酸铵法、EDTA-Na2法及尿素法提取的琼脂糖在得率和品质上的差异。结果表明，4种方法提取的琼脂糖在得率和品质方面都具有较大的差别；EDTA-Na2法琼脂糖的得率最高，为62.3%；聚乙二醇法琼脂糖中的硫酸根和灰分含量最低，分别为1.39%和0.42%；尿素法琼脂糖的结晶紫电泳性能最好，其电泳长度为8.6cm；EDTA-Na2法琼脂糖的聚乙二醇电泳性能最好，电泳长度为3.9cm。随着聚乙二醇法提取次数的增加，琼脂糖纯度和质量显著提高。用聚乙二醇法提取三次所获得的琼脂糖的各项质量指标比其他3种方法获得的琼脂糖都好。

Abstract:

Study on agarose processing method is important for realizing domestic agarose production. Four methods, including polyethylene glycol, ammonia sulfate, EDTA-Na2 and urea methods were used to extract agarose. The four methods showed significant difference in agarose productivity and quality, among which the EDTA-Na2 method obtained the highest agarose productivity of 62.3%, the polyethylene glycol method produced agarose containing the least sulfate (1.39%) and ash (0.42%), the urea method produced agarose with the best electrophoresis characteristics of crystal violet which showed an electrophoresis length of 8.6 cm, while the EDTA-Na2 method produced agarose with the best electrophoresis characteristics of polyethylene glycol which displayed an electrophoresis length of 3.9 cm. With the polyethylene glycol method, the longer the extraction time, the greater the purity and quality of the agarose. Triple polyethylene glycol extraction obtained better agarose than those of other methods. These results would provide parameters for optimizing agarose production methods.