饥饿和恢复投喂对许氏平鲉幼鱼消化酶活性的影响

The activities of digestive enzymes in Sebastes schlegeli juvenile after starvation and refeeding

关键词:  许氏平鲉幼鱼  饥饿  恢复投喂  消化酶活性

Key words:  Sebastes schlegeli juvenile  Starvation  Refeeding  Activities of digestive enzymes

摘要:

在水温为17.6±0.2℃条件下，研究了饥饿和恢复投喂对许氏平鲉幼鱼肝脏和肠主要消化酶活性的影响。实验分4组,每组设3个平行，其中A组为对照组，即在实验过程中正常投喂；B组饥饿5d恢复投喂50d；C组饥饿10d，恢复投喂45d；D组饥饿15d，恢复投喂40d。实验结果表明，蛋白酶、胰蛋白酶随饥饿时间的延长其活性下降明显，脂肪酶活性虽有下降趋势但不明显，而淀粉酶则在饥饿初期活性大幅度上升；恢复投喂后除了肠蛋白酶和肝胰蛋白酶外，其他消化酶活性均达到对照组水平。

Abstract:

In this study, the specific activity of digestive enzymes of Sebastes schlegeli juvenile subjected to starvation and refeeding was determined at 17.6±0.2℃. The experiment included four treatments, and within each treatment there were three replicates. Treatment A was the control group, which were fed normally during the process of the study. Treatment B experienced 5d food deprivation and 50d refeeding, Treatment C experienced 10d food deprivation and 45d refeeding. Treatment D experienced 15d food deprivation and 40d refeeding. The experimental results showed that the activities of protease and trypsin decreased obviously during the starvation; the activity of lipase went on a downward trend, which was not significant; the activity of amylase increased greatly during the early days of starvation. After refeeding， except protease in intestines and trypsin in liver, activities of all other digestive enzymes returned to the normal level.