| 摘要: |
| 利用通用引物对5个栉江珧野生群体(山东长岛、山东日照、山东文登、广东湛江和海南海口) 28S rRNA 和COI 基因片段进行扩增测序，分别得到983bp和623bp的片段，基于28S rRNA序列分析系统发生的结果表明，栉江珧与Atrina vexillumju具有较近的遗传关系。基于COI基因序列的遗传多样性分析结果显示，山东文登群体具有最高的遗传多样性水平。AMOVA分析显示，群体遗传分化系数为0.132 3(P<0.001)，说明栉江珧遗传变异主要来源于群体内的变异。由28S rRNA和COI基因聚类分析结果推测，由于地理隔离，我国栉江珧南北方群体可能早已分化为不同亚种。这些数据为我国栉江珧种质资源保护和利用补充了分子生物学资料。 |
| 关键词: 栉江珧 28S rRNA COI 遗传多样性 系统进化 |
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| 基金项目:山东省科技发展计划项目（2010GHY10513)、山东省自然科学基金项目（ZR2009DQ006)、青岛市成果转化项目（10-3-4-17-chg)和市南区2011年第二批科技发展基金项目（2011-5-023-QT） |
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| The genetic diversity and phylogenetic studies of five populations of Atrina pectinta in China |
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| Abstract: |
| Mitochondrial COI gene fragments and ribosomal 28S rRNA of Atrina pectinta sampled from five locations including three in Shandong (Changdao, Wendeng, and Rizhao), one Guangdong (Zhanjiang) and one in Hainan (Haikou) were amplified with universal primers, and 623bp and 983bp nucleotide sequences were obtained, respectively. Phylogenetic studies of Pinnidae based on 28S rRNA partial sequences showed that A. pectinta had a close genetic relationship with Atrina vexillumju. Genetic diversity of the five populations based on COI partial sequences demonstrated that the Wendeng population had the highest level of genetic diversity. The fixation indices (Fst) analyzed by AMOVA was 0.132 3 (P<0.001), indicating that the genetic variation of A. pectinta was mainly from genetic fixation inter-populations. Cluster analysis based on 28S rRNA and COI showed that A. pectinta in China might have differentiated into different subspecies. These findings would provide molecular biological data for the protection and utilization of A. pectinta resources in China. |
| Key words: Atrina pectinta 28S rRNA COI Genetic diversity Phylogeny |
