中国对虾不同凝集素基因应答WSSV侵染的表达差异

Expression of seven kinds of lectins after WSSV chanllenge in Chinese shrimp Fenneropenaeus chinensis

关键词:  中国对虾  白斑综合征病毒  细胞凝集素  表达差异

Key words:  Fenneropenaeus chinensis  WSSV  Lectin  Difference in gene express

摘要:

本研究探讨了7种细胞凝集素基因在中国对虾中应答白斑综合征病毒（WSSV）感染上的生物学功能差异。结果显示，LT1（DQ167572.1）、LT2（EU834289.1）、LT3（EU834292.1）和LT5（EU834290.1）是肝胰腺组织特异、受WSSV感染诱导表达的基因，其他3种基因主要在肌肉、肠和腮中表达，与WSSV感染诱导无明显相关；在应答WSSV感染不同时间段的表达特征上，7种凝集素基因各不相同;在各组织中的表达量上，方差分析表明7种凝集素基因除在肝胰腺中的表达上存在显著差异外，其他组织中不存在显著差异。这些结果显示这7个凝集素基因在应答WSSV侵染的表达特征上存在较大差异，推测其在中国对虾体内免疫防御功能上的作用也各不相同。

Abstract:

According to the gene sequences, the lectins in the genome of Chinese shrimp Fenneropenaeus chinensis, could be assorted into seven kinds of genes. In order to explore the expression differences among the seven lectins after WSSV challenge, real-time quantitative PCR was used to detect the expression profiles of these lectins at different time after WSSV challenge in Chinese shrimp. The results showed that, of the seven lectins, LT1(DQ167572.1), LT2(EU834289.1), LT3(EU834292.1) and LT5(EU834290.1) displayed hepatopancreas-specific expression profiles, and were WSSV-induced genes, while the other genes were mainly expressed in gills, intestines and muscles, and were non-WSSV-induced genes. The expression profiles of seven detected genes at different time after WSSV challenge were different with each other. Additionally, the analysis of variance (ANOVA) indicated that no significant difference was found at the expression level of seven lectins in gills, intestines and muscles, except hepatopancreases. These results showed that the biology function of the seven lectins were different from each other in Chinese shrimp F. chinensis.