褐牙鲆(♀)×犬齿牙鲆(♂)杂交子代及亲本群体形态和外周血红细胞DNA含量

The morphologic characters and DNA contents of peripheral blood erythrocytes of Paralichthys dentatus(♂), Paralichthys olivaceus(♀) and their crossbreed F1 progenies

关键词:  犬齿牙鲆  褐牙鲆  杂交子代  形态  DNA含量

Key words:  Paralichthys olivaceus  Paralichthys dentatus  Crossbreed F1 generation  Morphologic characters  DNA contents

摘要:

2006～2007年进行了犬齿牙鲆Paralichthys dentatus(♂)×褐牙鲆P.olivaceus(♀)杂交，获得杂交子代苗种养殖至全长27～33 cm时随机取样，测定形态学特征。与父、母本相同规格成鱼相比，杂交子代的有眼侧体色和花纹与父、母本都存在较大差异，是区分杂交子代最突出的特征。杂交子代的多数可比、可量性状同父、母本相近，但在体长/总高比等方面表现出较明显的杂交优势。聚类分析表明,杂交子代的表型与犬齿牙鲆更为接近。尾静脉采血，使用流式细胞仪检测杂交子代和父、母本外周血红细胞的DNA含量，发现杂交子代、犬齿牙鲆和褐牙鲆的DNA含量分别为1.51±0.02、1.50±0.04、1.43±0.16 pg，杂交子代的DNA含量与犬齿牙鲆接近，较褐牙鲆略高，表明杂交子代为二倍体。

Abstract:

The crossbreed F1 progenies of Paralichthys dentatus♂×P. olivaceus♀ have been cultured since 2006 in indoor tanks. The F1 fish (27~33cm in total length) and their parents were randomly sampled for morphological measurements. There were no significant difference for morphological parameters between F1 fish and their parents. In addition, heterosis was manifested by ratio of body length and total height. The pigmentation and stripes on the body surface of F1 were different from their parents. Peripheral blood was sampled from F1 progenies and their parents respectively by withdrawing from tail vein. The DNA contents of erythrocytes in peripheral blood were determined using PARTEC Cell Counter Analyzer(CCA-Ⅱ). The DNA contents of F1 fish, P. dentatus and P. olivaceus were 1.51±0.02pg, 1.50±0.04pg and 1.43±0.16pg respectively. The DNA contents of F1 fish were similar to their male parents, P. dentatus, indicating that the heredity of F1 fish may bear more close relationship with P. dentatus. Meantime, the results showed that the F1 fish were diploid.