不同毒力溶藻弧菌脂多糖的多态性研究

The polymorphism of lipopolysaccharides isolated from Vibrio alginolyticus strains with different virulence

关键词:  溶藻弧菌  脂多糖  毒力  多态性

Key words:  Vibrio alginolyticus  Lipopolysaccharides (LPS)  Virulence  Polymorphism

摘要:

提取了10株不同毒力的溶藻弧菌脂多糖，其中HN08811、HN08155、HN08813、HN08335、HN07006为毒力菌株，HN08805、HN08307、HN08156、HN08801和TG06003为非毒力菌株。分别取10 μg进行SDS-PAGE电泳。结果表明，不同毒力溶藻弧菌脂多糖的电泳条带存在较丰富的多态性，在类脂质A区段（分离胶的上半部分），不同菌株间图谱呈片状堆积，多态性难以分辨；在抗原多糖O-特异性侧链条带区段（分离胶的下半部分），除HN07006外，毒力菌株的条带清晰，数目在7~9条之间，所有非毒力菌株产生细而密集的可辨条带。本文为一类遗传型的溶藻弧菌毒力菌株的快速鉴别提供了一种新的分子分型指标，也为溶藻弧菌的血清型分型提供了直接理论依据。

Abstract:

Lipopolysaccharides (LPS) were extracted from ten Vibrio alginolyticus strains with different virulence. Five of them were virulent strains, named as HN08811, HN08155, HN08813, HN08335, and HN07006 and the others were non-virulent strains, named as HN08805, HN08307, HN08232, HN08201, and TG06003. The results of SDS-PAGE (sodium dodecyl sulfate polyacrylamide polyacrylamide gel electrophoresis) with 10 μg LPS showed that LPS of V. alginolyticus strains with different virulence had abundant polymorphism. The polymorphism of lipid A section of each LPS was difficult to distinguish. However, for the O-antigen section, 7~8 clear bands of LPSs from virulent strains were observed except for HN07006. LPSs from all the non-virulent strains had discernable slender and intensive bands. This method not only creates a new molecular typing method for rapid identification of virulent strains of V. alginolyticus, but also provides scientific theoretical data for the analysis of serotype of V. alginolyticus.