荣福海带中内源植物激素含量的动态变化研究

Variations of endogenous hormones in Rongfu Laminaria saccharia at different development stage

关键词:  高效液相色谱  荣福海带  内源植物激素

Key words:  HPLC  Rongfu Laminaria saccharia  Endogenous hormones

摘要:

以荣福海带为材料，采用高效液相色谱法紫外检测不同时期和不同部位海带中内源植物激素含量的变化。结果表明，在海带生长的幼嫩期、厚成期和成熟期，吲哚乙酸(IAA)和脱落酸(ABA)呈递增的趋势；玉米素(ZT)在海带成熟期达到最大值，厚成期含量最少；吲哚丁酸(IBA)在厚成期达到最大值，幼嫩期含量最少。同一生长阶段的海带体内植物激素纵向分布规律为：海带尖部的IAA含量较高，中带部的ZT含量明显较高，而基部的IBA和ABA的含量较高。

Abstract:

The variations of endogenous hormones in different periods and different parts of Rongfu Laminaria saccharia were analyzed by high performance liquid chromatography coupled with UV detector. It was found that the content of indole-3-acetic acid (IAA) and abscisic acid (ABA) increased significantly in three important periods of L. japonica (juvenile,grow-out,mature). The content of zeatin (ZT) reached the highest in the mature period,and the lowest in the grow-out period. The content of indolebutyric acid (IBA) reached the highest in the grow-out period,and the lowest in the juvenile period. At each development stage,different plant hormones were found at the highest contents in different parts of the L. saccharia blade; IAA showed the highest content in the top,while the highest contents of ZT was found in the middle,and both IBA and ABA were found in the bottom.